College of New Energy and Environment, Jilin University, Changchun, 130012, China.
College of New Energy and Environment, Jilin University, Changchun, 130012, China; Key Laboratory of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun, 130012, China.
Sci Total Environ. 2022 Oct 20;844:157177. doi: 10.1016/j.scitotenv.2022.157177. Epub 2022 Jul 6.
Indoor dust is the main carrier of indoor pollutants, especially dust mite allergens and bacteria, they can trigger asthma, rhinitis, eczema and other allergic diseases. However, the interactions between dust mite allergens and bacterial communities in different types of indoor dust are not clear. The study focused on particulate and flocculent fibrous dust, explored the concentrations of Der p 1 (Dermatophagoides pteronyssinus) and Der f 1 (D. farinae) in 46 households in Changchun and their environmental influences, characterized the bacterial communities by high-throughput sequencing, and the interactions between Der p 1, Der f 1 and bacterial communities were explored. The results showed that Der p 1 and Der f 1 tended to accumulate more in flocculent fibrous dust, and Der p 1 predominated in the indoor dust samples. The floor height, years of housing occupancy and the living areas all affected the concentrations of dust mite allergens. In bacterial community, Proteobacteria, Firmicutes and Actinobacteria were leading phyla in the two types of dust. Kocuria, Blastococcus and Massilia were dominating genera in particulate dust and Acinetobacter, Lactobacillus, Corynebacterium_1 were dominating genera in flocculent fibrous dust. The overall diversity and species richness of bacteria in particulate dust were significantly higher than those in flocculent dust (p < 0.001). The living area was an important environmental factor affecting the bacterial community in flocculent fibrous dust (p < 0.01). The interaction between the relative abundance of Proteobacteria, Firmicutes and Actinobacteria and dust mite allergen concentrations significantly differed between the two dust types, indicating that bacteria could be used both as food and to establish symbiotic relationships with household dust mites (HDMs) hosts and provide nutrition.
室内灰尘是室内污染物的主要载体，尤其是尘螨过敏原和细菌，它们会引发哮喘、鼻炎、湿疹等过敏疾病。然而，不同类型室内灰尘中尘螨过敏原和细菌群落之间的相互作用尚不清楚。本研究聚焦于颗粒状和絮状纤维灰尘，探究了长春 46 户家庭中 Der p 1（屋尘螨）和 Der f 1（粉尘螨）的浓度及其环境影响，通过高通量测序对细菌群落进行了特征描述，并探究了 Der p 1、Der f 1 与细菌群落的相互作用。结果表明，Der p 1 和 Der f 1 更倾向于在絮状纤维灰尘中积累，且室内灰尘样本中 Der p 1 占主导地位。楼层高度、住房居住年限和生活区域均影响尘螨过敏原的浓度。在细菌群落中，变形菌门、厚壁菌门和放线菌门是两种灰尘中的主要门。颗粒状灰尘中以 Kocuria、Blastococcus 和 Massilia 为主导属，絮状纤维灰尘中以 Acinetobacter、Lactobacillus 和 Corynebacterium_1 为主导属。颗粒状灰尘中的细菌整体多样性和丰富度明显高于絮状灰尘（p<0.001）。生活区域是影响絮状纤维灰尘中细菌群落的重要环境因素（p<0.01）。变形菌门、厚壁菌门和放线菌门相对丰度与尘螨过敏原浓度之间的相互作用在两种灰尘类型之间存在显著差异，表明细菌既可以作为食物，也可以与家庭尘螨（HDM）宿主建立共生关系并提供营养。
