Hubert Jan, Sopko Bruno, Głowska-Patyniak Eliza
Czech Agrifood Research Center - CARC, Prague, Prague 6 - Ruzyne, Czechia.
Department of Animal Morphology, Faculty of Biology, Adam Mickiewicz University in Poznan, Poznan, Poland.
Environ Microbiol Rep. 2025 Aug;17(4):e70142. doi: 10.1111/1758-2229.70142.
Storage mites consume stored products in interaction with environmental microorganisms, resulting in the destruction of infested food and providing specific odours. Here we simulated the effect of mite grazing on oat flakes. Spent growth medium (SPGM) was obtained from seven mite cultures and mixed with oat flakes as the source of faeces and microbes. SPGM-treated diets were offered to 4 mite cultures. The microbiomes were analysed using sequencing of V4_16S_DNA. Mite growth tests, food preferences, and microbiome changes were observed in correlation with SPGM type and mite cultures. The microbiome consisted of 41 OTUs belonging to mite-associated bacteria and faeces bacteria. The composition of the microbiome depends more on the source of SPGM than on mite culture. The SPGM diet accelerated mite population growth and influenced mite food choice, although the effect was dependent on both types of SPGM and mite culture. Kocuria, Brevibacterium, Virgibacillus, and Staphylococcus profiles in SPGM added into diets showed positive correlations to mite population growth. The Kocuria profile in the bodies of mites was positively correlated with mite population growth. The results showed that mites are influenced by SPGM-treated diets, and mite feeding influences the environmental microbiome. The most beneficial was the mite interaction with Kocuria.
储藏螨与环境微生物相互作用，消耗储存的产品，导致受侵染的食物遭到破坏，并产生特定气味。在此，我们模拟了螨类啃食燕麦片的影响。从七种螨类培养物中获取用过的生长培养基（SPGM），并将其与作为粪便和微生物来源的燕麦片混合。将经SPGM处理的饲料提供给4种螨类培养物。使用V4_16S_DNA测序分析微生物群落。观察到螨类生长测试、食物偏好和微生物群落变化与SPGM类型和螨类培养物之间的相关性。微生物群落由41个属于螨类相关细菌和粪便细菌的操作分类单元（OTU）组成。微生物群落的组成更多地取决于SPGM的来源，而非螨类培养物。尽管这种影响取决于SPGM类型和螨类培养物两者，但SPGM饲料加速了螨类种群的增长并影响了螨类的食物选择。添加到饲料中的SPGM中的科氏杆菌属、短杆菌属、 Virgibacillus和葡萄球菌属谱与螨类种群增长呈正相关。螨类体内的科氏杆菌属谱与螨类种群增长呈正相关。结果表明，螨类受到经SPGM处理的饲料的影响，而螨类取食会影响环境微生物群落。螨类与科氏杆菌属的相互作用最为有益。
