Gaeta Natália C, de Sá Guimarães Ana Marcia, Timenetsky Jorge, Clouser Stephanie, Gregory Lilian, Ganda Erika
Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Laboratory of Bacterial Zoonosis, Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Vet Res Commun. 2022 Dec;46(4):1311-1318. doi: 10.1007/s11259-022-09972-x. Epub 2022 Jul 8.
Mycoplasma ovipneumoniae is an important etiological agent of sheep respiratory disease worldwide. Here, we describe the first isolation and draft genome sequence of M. ovipneumoniae strain USP-BR2017 retrieved from tracheobronchial lavage of a sheep showing clinical signs of respiratory disease in the Rio de Janeiro State, Brazil. The culture of tracheobronchial lavage resulted in glucose-fermenting fried egg colonies, which were identified as M. ovipneumoniae by polymerase chain reaction. The genome was sequenced using the Illumina NextSeq 2000 and de novo assembled using SPAdes. The genome of the sequenced organism presented an approximate size of 1,122,253 bp. The annotation revealed 773 coding DNA sequences (CDSs), 806 genes, three rRNAs, and 30 tRNAs. Data analysis revealed M. ovipneumoniae strain USP-BR2017 contains a few virulence genes, including the hemolysing C gene (hlyC). In addition, strain USP-BR2017 showed high identity over the 16S rRNA gene with other sheep isolates from China and United States. This first description of M. ovipneumoniae in diseased Brazilian sheep demonstrates the importance of continuous surveillance and diagnostics of pathogens causing respiratory disease in sheep in Brazil.
绵羊肺炎支原体是全球绵羊呼吸道疾病的重要病原体。在此，我们描述了从巴西里约热内卢州一只出现呼吸道疾病临床症状的绵羊的气管支气管灌洗样本中分离出的绵羊肺炎支原体USP - BR2017菌株及其基因组草图序列。气管支气管灌洗样本培养后形成了葡萄糖发酵的煎蛋样菌落，通过聚合酶链反应鉴定为绵羊肺炎支原体。使用Illumina NextSeq 2000对基因组进行测序，并使用SPAdes进行从头组装。测序菌株的基因组大小约为1,122,253 bp。注释显示有773个编码DNA序列（CDS）、806个基因、3个rRNA和30个tRNA。数据分析表明，绵羊肺炎支原体USP - BR2017菌株含有一些毒力基因，包括溶血C基因（hlyC）。此外，USP - BR2017菌株在16S rRNA基因上与来自中国和美国的其他绵羊分离株具有高度同源性。巴西患病绵羊中绵羊肺炎支原体的这一首次描述证明了巴西持续监测和诊断引起绵羊呼吸道疾病病原体的重要性。
