College of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, 530001, Guangxi, China.
BMC Microbiol. 2023 Aug 14;23(1):220. doi: 10.1186/s12866-023-02964-0.
Mycoplasma ovipneumoniae is a critical pathogen that causes respiratory diseases that threaten Caprini health and cause economic damage. A genome-wide study of M. ovipneumoniae will help understand the pathogenic characteristics of this microorganism.
Toxicological pathology and whole-genome sequencing of nine M. ovipneumoniae strains isolated from goats were performed using an epidemiological survey. These strains exhibited anterior ventral lung consolidation, typical of bronchopneumonia in goats. Average nucleotide identity and phylogenetic analysis based on whole-genome sequences showed that all M. ovipneumoniae strains clustered into two clades, largely in accordance with their geographical origins. The pan-genome of the 23 M. ovipneumoniae strains contained 5,596 genes, including 385 core, 210 soft core, and 5,001 accessory genes. Among these genes, two protein-coding genes were annotated as cilium adhesion and eight as paralog surface adhesins when annotated to VFDB, and no antibiotic resistance-related genes were predicted. Additionally, 23 strains carried glucosidase-related genes (ycjT and group_1595) and glucosidase-related genes (atpD_2), indicating that M. ovipneumoniae possesses a wide range of glycoside hydrolase activities.
The population structure and genomic features identified in this study will facilitate further investigations into the pathogenesis of M. ovipneumoniae and lay the foundation for the development of preventive and therapeutic methods.
绵羊肺炎支原体是一种重要的病原体，可引起威胁绵羊 Caprini 健康并造成经济损失的呼吸道疾病。对绵羊肺炎支原体进行全基因组研究有助于了解该微生物的致病特性。
采用流行病学调查方法，对从山羊中分离出的 9 株绵羊肺炎支原体进行了毒理学病理学和全基因组测序。这些菌株表现出前腹侧肺实变，这是山羊支气管肺炎的典型特征。基于全基因组序列的平均核苷酸同一性和系统发育分析表明，所有绵羊肺炎支原体菌株分为两个分支，这与它们的地理起源基本一致。23 株绵羊肺炎支原体的泛基因组包含 5596 个基因，包括 385 个核心基因、210 个软核心基因和 5001 个辅助基因。在这些基因中，有两个编码蛋白的基因被注释为纤毛黏附蛋白，有八个被注释为平行表面黏附素，VFDB 预测没有抗生素抗性相关基因。此外，23 株菌携带糖苷酶相关基因（ycjT 和 group_1595）和糖苷酶相关基因（atpD_2），表明绵羊肺炎支原体具有广泛的糖苷水解酶活性。
本研究中确定的种群结构和基因组特征将有助于进一步研究绵羊肺炎支原体的发病机制，并为预防和治疗方法的开发奠定基础。
