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海虫()水提取物和蛋白质水解物的抗氧化活性

Antioxidant Activities of Aqueous Extracts and Protein Hydrolysates from Marine Worm Hechong ().

作者信息

Zhang Wenxia, Wang Zexiong, Ganesan Kumar, Yuan Yingzhi, Xu Baojun

机构信息

Programme of Food Science and Technology, BNU-HKBU United International College, Zhuhai 519087, China.

School of Chinese Medicine, Li Ka Shing (LKS) Faculty of Medicine, University of Hong Kong, Sassoon Road, Hong Kong 999077, China.

出版信息

Foods. 2022 Jun 22;11(13):1837. doi: 10.3390/foods11131837.

Abstract

Hechong () is an edible marine worm widely distributed in the estuary area. The objective of this study is to determine the antioxidant activities of extracts and protein hydrolysates from Hechong. Results showed that the aqueous extracts of steamed Hechong had the highest antioxidant values using the methods of DPPH, ABTS, and FRAP testing (76.29 μmol TE/g, 181.04 μmol TE/g, and 10.40 mmol Fe/100 g, respectively). Furthermore, protein hydrolysates of Hechong were observed significant antioxidant activities when compared to crude Hechong. The purification was carried out by DEAE-52 cellulose and Sephadex G-100 column chromatography. The microspatial structure of glycoprotein showed fibrous shapes and cracks with uniform distribution. The study has concluded that the extract and protein hydrolysates of Hechong have significant antioxidant activities, which is merited to be further investigated in the food and pharmaceutical fields.

摘要

沙虫是一种广泛分布于河口地区的可食用海洋蠕虫。本研究的目的是测定沙虫提取物和蛋白水解物的抗氧化活性。结果表明,采用DPPH、ABTS和FRAP测试方法,清蒸沙虫的水提取物具有最高的抗氧化值(分别为76.29 μmol TE/g、181.04 μmol TE/g和10.40 mmol Fe/100 g)。此外,与粗沙虫相比,沙虫蛋白水解物具有显著的抗氧化活性。通过DEAE - 52纤维素和Sephadex G - 100柱色谱进行纯化。糖蛋白的微观空间结构呈纤维状且有均匀分布的裂缝。该研究得出结论,沙虫提取物和蛋白水解物具有显著的抗氧化活性,值得在食品和制药领域进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/33c9/9265576/352eeea639e9/foods-11-01837-g001.jpg

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