Thungmungmee Suradwadee, Sritularak Boonchoo, Wisidsri Nakuntwalai, Kanana Nattakan, Nimmano Nattika
Faculty of Integrative Medicine, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand.
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok, Thailand.
Nanotechnol Sci Appl. 2025 Mar 24;18:157-171. doi: 10.2147/NSA.S508582. eCollection 2025.
The study aimed to investigate in vitro anti-aging activities of 29 spp. and develop and characterize microemulsions (MEs) for topical application.
Antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), HO scavenging, and ferric reducing antioxidant power (FRAP) assays. The anti-collagenase (MMP-1 and MMP-2) and anti-elastase activities were also evaluated. Cytotoxicity and human intracellular reactive oxygen species (ROS) levels were determined using resazurin reduction and 2',7'-dichlorofluorescin diacetate (DCFDA) assays, respectively. extract-loaded microemulsion (DKME) was then prepared and optimized. The stability of DKME was studied using a heating-cooling cycle.
appeared to be the best candidate anti-aging agent because of its antioxidant, anti-collagenase, and anti-elastase activities. The extract was safe for human skin cells at a concentration of 6.25-100 μg/mL. It also decreased the intracellular ROS-induced ultraviolet B (UVB) irradiation compared to that in the control. DKME comprising Tween 80:ethanol (5:1), water, and isononyl isononanoate showed a suitable appearance, droplet size, polydisperse index, zeta potential, pH, and viscosity. This formulation demonstrated desirable physical and chemical stability, with non-cytotoxic effects.
DKME is considered a promising anti-aging product. However, an in vivo study of this optimized formulation might be evaluated in further study for anti-aging purposes.
本研究旨在调查29种植物的体外抗老化活性，并开发和表征用于局部应用的微乳剂（MEs）。
使用2,2-二苯基-1-苦基肼（DPPH）、羟基自由基清除和铁还原抗氧化能力（FRAP）测定法测定抗氧化活性。还评估了抗胶原酶（MMP-1和MMP-2）和抗弹性蛋白酶活性。分别使用刃天青还原和2',7'-二氯荧光素二乙酸酯（DCFDA）测定法测定细胞毒性和人细胞内活性氧（ROS）水平。然后制备并优化了提取物负载微乳剂（DKME）。使用加热-冷却循环研究DKME的稳定性。
因其抗氧化、抗胶原酶和抗弹性蛋白酶活性，似乎是最佳的抗老化剂候选物。该提取物在浓度为6.25-100μg/mL时对人皮肤细胞安全。与对照组相比，它还降低了细胞内ROS诱导的紫外线B（UVB）照射。由吐温80:乙醇（5:1）、水和异壬酸异壬酯组成的DKME具有合适的外观、液滴大小、多分散指数、zeta电位、pH和粘度。该制剂表现出理想的物理和化学稳定性，且无细胞毒性作用。
DKME被认为是一种有前途的抗老化产品。然而，这种优化制剂的体内研究可能需要在进一步的抗老化研究中进行评估。
