Chen Xiaoyun, Yu Huiru, Wang Pengfei, Peng Cheng, Wang Xiaofu, Xu Xiaoli, Xu Junfeng, Liang Jingang, Li Liang
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321001, China.
Foods. 2022 Jun 25;11(13):1888. doi: 10.3390/foods11131888.
is a novel glyphosate herbicide-resistant gene that has been transferred to various crops such as soybean, corn, cotton, and rice. Here, we developed a gene-specific digital Polymerase Chain Reaction (dPCR) detection method for absolute quantitative analysis of , and characterized certified reference materials (CRM) using ZUTS-33 soybean powder as the candidate material. Stability tests of matrix CRMs demonstrate that these CRMs can be stored stably for 6 months and transported for 10 days at room temperature and withstand summer high temperatures (below 60 °C). CRM characterization is based on the copy number ratio of to . Eight qualified laboratories independently validated the CRM using dPCR method, with a measurement of 0.98 (copy/copy) and an extended uncertainty of 0.08 (copy/copy). The matrix CRM described here may be used for qualitative and quantitative testing, method evaluation, laboratory quality control, and other related fields.
是一种新型抗草甘膦除草剂基因，已被转入大豆、玉米、棉花和水稻等多种作物中。在此，我们开发了一种针对该基因的数字聚合酶链反应（dPCR）检测方法用于其绝对定量分析，并以ZUTS - 33大豆粉作为候选材料对认证参考物质（CRM）进行了特性鉴定。基质CRM的稳定性测试表明，这些CRM在室温下可稳定储存6个月，运输10天，并能承受夏季高温（低于60°C）。CRM的特性鉴定基于该基因与[具体对比基因]的拷贝数比。八个合格实验室使用dPCR方法对CRM进行了独立验证，测量值为0.98（拷贝/拷贝），扩展不确定度为0.08（拷贝/拷贝）。这里描述的该基质CRM可用于定性和定量检测、方法评估、实验室质量控制及其他相关领域。
