Ralaivao Mathis, Lucas Jade, Rocha Fernando, Estevinho Berta N
LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
ENSCM-Ecole Nationale Supérieure de Chimie de Montpellier, 8 Rue de l'Ecole Normale, CEDEX 5, 34296 Montpellier, France.
Foods. 2022 Jul 5;11(13):1990. doi: 10.3390/foods11131990.
Epigallocatechin gallate (EGCG) is a catechin and one of the most abundant polyphenols in green tea, and it is under research for its potential benefit to human health and for its potential to be used in disease treatments, such as for cancer. However, the effectiveness of polyphenols depends on preserving their bioactivity, stability, and bioavailability. The EGCG was microencapsulated by a spray-drying process, using different biopolymers as encapsulating agents (gum arabic, modified chitosan and sodium alginate), in order to overcome some of the limitations of this compound. The microparticles showed a diameter around 4.22 to 41.55 µm (distribution in volume) and different morphologies and surfaces, depending on the encapsulating agent used. The EGCG release was total, and it was achieved in less than 21 min for all the formulations tested. The EGCG encapsulation efficiency ranged between 78.5 and 100.0%. The release profiles were simulated and evaluated using three kinetic models: Korsmeyer-Peppas (R: 0.739-0.990), Weibull (R: 0.963-0.994) and Baker-Lonsdale (R: 0.746-0.993). The Weibull model was the model that better adjusted to the experimental EGCG release values. This study proves the success of the EGCG microencapsulation, using the spray-drying technique, opening the possibility to insert dried EGCG microparticles in different food and nutraceutical products.
表没食子儿茶素没食子酸酯（EGCG）是一种儿茶素，也是绿茶中含量最丰富的多酚之一，目前正在研究其对人体健康的潜在益处以及用于疾病治疗（如癌症治疗）的潜力。然而，多酚的有效性取决于保持其生物活性、稳定性和生物利用度。为了克服该化合物的一些局限性，采用喷雾干燥工艺，使用不同的生物聚合物（阿拉伯胶、改性壳聚糖和海藻酸钠）对EGCG进行微胶囊化。根据所使用的包囊剂不同，微粒的直径约为4.22至41.55 µm（体积分布），且具有不同的形态和表面。所有测试配方的EGCG释放均完全，且在不到21分钟内即可实现。EGCG的包封效率在78.5%至100.0%之间。使用三种动力学模型对释放曲线进行模拟和评估：Korsmeyer-Peppas模型（R：0.739 - 0.990）、Weibull模型（R：0.963 - 0.994）和Baker-Lonsdale模型（R：0.746 - 0.993）。Weibull模型是最能拟合实验EGCG释放值的模型。本研究证明了采用喷雾干燥技术对EGCG进行微胶囊化的成功，为将干燥的EGCG微粒应用于不同食品和营养保健品开辟了可能性。
