Nofima-Norwegian Institute of Food, Fisheries and Aquaculture Research, P.O. Box 210, NO-1431 Ås, Norway.
Center for Materials Science and Nanotechnology (SMN), Department of Chemistry, University of Oslo, P.O. Box 1033, Blindern, NO-0315 Oslo, Norway.
Int J Mol Sci. 2022 Jun 24;23(13):7032. doi: 10.3390/ijms23137032.
Essential oils and their active components have been extensively reported in the literature for their efficient antimicrobial, antioxidant and antifungal properties. However, the sensitivity of these volatile compounds towards heat, oxygen and light limits their usage in real food packaging applications. The encapsulation of these compounds into inorganic nanocarriers, such as nanoclays, has been shown to prolong the release and protect the compounds from harsh processing conditions. Nevertheless, these systems have limited shelf stability, and the release is of limited control. Thus, this study presents a mesoporous silica nanocarrier with a high surface area and well-ordered protective pore structure for loading large amounts of natural active compounds (up to 500 mg/g). The presented loaded nanocarriers are shelf-stable with a very slow initial release which levels out at 50% retention of the encapsulated compounds after 2 months. By the addition of simulated drip-loss from chicken, the release of the compounds is activated and gives an antimicrobial effect, which is demonstrated on the foodborne spoilage bacteria and the potentially pathogenic bacteria . When the release of the active compounds is activated, a ≥4-log reduction in the growth of and a 2-log reduction of is obtained, after only one hour of incubation. During the same one-hour incubation period the dry nanocarriers gave a negligible inhibitory effect. By using the proposed nanocarrier system, which is activated by the food product itself, increased availability of the natural antimicrobial compounds is expected, with a subsequent controlled antimicrobial effect.
精油及其有效成分在文献中被广泛报道，具有高效的抗菌、抗氧化和抗真菌特性。然而，这些挥发性化合物对热、氧和光敏感，限制了它们在实际食品包装应用中的使用。将这些化合物封装到无机纳米载体中，如纳米粘土中，已被证明可以延长释放时间并保护化合物免受苛刻的加工条件的影响。然而，这些系统的货架稳定性有限，释放的控制也有限。因此，本研究提出了一种具有高表面积和有序保护孔结构的介孔硅纳米载体，可用于负载大量天然活性化合物（高达 500mg/g）。所提出的负载纳米载体具有稳定的货架期，初始释放非常缓慢，在 2 个月后，封装化合物的保留率达到 50%。通过添加模拟鸡肉滴液损失，化合物的释放被激活并产生抗菌作用，这在食源性腐败细菌和潜在的病原菌中得到了证明。当释放活性化合物时，仅需孵育 1 小时， 和 的生长就减少了≥4 个对数， 减少了 2 个对数。在相同的 1 小时孵育期间，干燥的纳米载体几乎没有抑制作用。通过使用由食品本身激活的提议的纳米载体系统，预计天然抗菌化合物的可用性会增加，从而产生后续的受控抗菌效果。
