Rao Akhil C, Venkatesh K Vijay, Nandini Vidyashree, Sihivahanan Dhanasekaran, Alamoudi Ahmed, Bahammam Hammam Ahmed, Bahammam Sarah Ahmed, Zidane Bassam, Bahammam Maha A, Chohan Hitesh, Albar Nassreen H, Yadalam Pradeep Kumar, Patil Shankargouda
Department of Conservative Dentistry and Endodontics, SRM Dental College and Hospital, Kattankulathur, Kanchipuram, Chennai 603203, India.
Department of Prosthodontics and Implantology, SRM Dental College and Hospital, Kattankulathur, Kanchipuram, Chennai 603203, India.
Materials (Basel). 2022 Jun 29;15(13):4567. doi: 10.3390/ma15134567.
Dental pulp treatment is the least intrusive procedure currently available for preserving the vitality of the pulp. Several studies are underway to improve the bioactivity of pulp capping materials. Tideglusib isa potent anti-inflammatory, antioxidant, and a regenerative drug developed against Alzheimer's disease and has been shown to be effective in the treatment of dental cavities. However, its bioactive properties encapsulated within the nanoparticles as a component of pulp capping material are largely unknown. In this study, tideglusib-loaded bioactive glass nanoparticles were synthesized (tideglusib-BgNPs) and mixed at various concentrations into the calcium silicate cement to testits physiomechanical and bioactivitiescompared with biodentine (control). The calcium silicate cement with 10wgt% tideglusib-BgNPs showed comparable physiomechanical properties to that of biodentine. Additionally, the assessment of cytotoxicity and bioactivity (cell proliferation, wound healing, and cell migration assays) showed increased bioactivity in terms of better wound healing, increased proliferation, and better migration of human dental pulp stem cells than biodentine. These findings suggest new opportunities to use tideglusib-BgNPs in pulp therapy.
牙髓治疗是目前可用于保留牙髓活力的侵入性最小的程序。目前正在进行多项研究以提高盖髓材料的生物活性。替格列脲是一种强效抗炎、抗氧化且针对阿尔茨海默病开发的再生药物，已被证明在治疗龋齿方面有效。然而，作为盖髓材料的一种成分，其包裹在纳米颗粒中的生物活性特性在很大程度上尚不清楚。在本研究中，合成了负载替格列脲的生物活性玻璃纳米颗粒（替格列脲 - BgNPs），并将其以不同浓度与硅酸钙水泥混合，以与生物牙本质（对照）相比测试其物理力学和生物活性。含有10重量%替格列脲 - BgNPs的硅酸钙水泥显示出与生物牙本质相当的物理力学性能。此外，细胞毒性和生物活性评估（细胞增殖、伤口愈合和细胞迁移试验）表明，与生物牙本质相比，人牙髓干细胞在伤口愈合更好、增殖增加和迁移更好方面表现出更高的生物活性。这些发现为替格列脲 - BgNPs在牙髓治疗中的应用提供了新的机会。
