Laboratory for Forensic Chemistry, Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Kraków, Poland.
Department of Forensic Medicine, Medical University in Wroclaw, 4 Jana Mikulicza-Radeckiego St., 50-345 Wrocław, Poland.
Molecules. 2022 Jun 27;27(13):4116. doi: 10.3390/molecules27134116.
The present investigation utilised the quick and easy SPME/LC-MS method to determine selected narcotic substances and their metabolites in whole blood. The study included qualitative analysis and validation of the method. Analytes were determined in the linearity range of 25−300 ng/mL. The precision during and between days (in general CV < 13.41%), and the LOD which results in between 0.36 and 11.08 ng/mL, and the LOQ between 1.20 and 36.90 ng/mL were investigated. The validation results obtained, as well as the results of subsequent in-laboratory tests, confirmed the applicability of the method in the analysis of blood samples. An attempt to apply the method to the analysis of bone marrow samples has yielded promising results; however, more detailed studies are needed in this area.
本研究利用快速简便的 SPME/LC-MS 方法测定全血中的选定麻醉物质及其代谢物。该研究包括方法的定性分析和验证。分析物在 25-300ng/mL 的线性范围内确定。在日内和日间（通常 CV<13.41%）的精密度以及在 0.36 至 11.08ng/mL 之间的检出限和在 1.20 至 36.90ng/mL 之间的定量限进行了研究。所获得的验证结果以及随后的实验室测试结果证实了该方法在分析血样中的适用性。尝试将该方法应用于骨髓样本的分析已取得了有希望的结果；然而，在这一领域还需要进行更详细的研究。
