Lan Yuning, Sun Ying, Liu Zhen, Wei Shouhui, Huang Hongjuan, Cao Yi, Li Wenyu, Huang Zhaofeng
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Agriculture, Northeast Agricultural University, Harbin 150030, China.
Plants (Basel). 2022 Jun 22;11(13):1645. doi: 10.3390/plants11131645.
Black grass ( Huds.) is a highly competitive weed in winter wheat fields of China. Due to repeated use of acetolactate synthase (ALS) inhibitors, many populations have evolved resistance to pyroxsulam in some wheat fields. Research was conducted to determine the molecular basis of herbicide resistance in the AH93 population. Whole-plant dose-response assay confirmed that the AH93 population was resistant to pyroxsulam with a resistance index of 4.2. Cross- and multiple-resistance assays indicated that the AH93 population was cross-resistant to mesosulfuron-methyl and multiple-resistant to pinoxaden. Sequencing of the and gene revealed that there was no target-site mutation in ALS, but Trp-2027-Cys and Cys-2088-Arg amino acid mutations in ACCase in the AH93 population. A malathion pretreatment study indicated that the AH93 population might have cytochrome P450-mediated herbicide metabolic resistance. This is the first report of pyroxsulam resistance in a multiple-resistant population in China, and the Cys-2088-Arg mutation is the first reported case of an ACCase mutant conferring herbicide resistance in .
看麦娘（Alopecurus aequalis Sobol.）是中国冬小麦田中的一种极具竞争力的杂草。由于乙酰乳酸合成酶（ALS）抑制剂的反复使用，一些麦田中的许多种群已对唑吡嘧磺隆产生了抗性。开展了研究以确定AH93种群中除草剂抗性的分子基础。整株剂量反应试验证实AH93种群对唑吡嘧磺隆具有抗性，抗性指数为4.2。交互抗性和多重抗性试验表明，AH93种群对甲基二磺隆具有交互抗性，对氯氟吡啶酯具有多重抗性。对ALS和ACCase基因进行测序发现，AH93种群的ALS中没有靶标位点突变，但ACCase中存在色氨酸-2027-半胱氨酸和半胱氨酸-2088-精氨酸氨基酸突变。一项马拉硫磷预处理研究表明，AH93种群可能具有细胞色素P450介导的除草剂代谢抗性。这是中国首次报道多抗性看麦娘种群对唑吡嘧磺隆的抗性，且半胱氨酸-2088-精氨酸突变是首次报道的ACCase突变赋予看麦娘除草剂抗性的案例。
