Peng Yi, Raj Nikhil, Strasser Juliette W, Crooks Richard M
Department of Chemistry, The University of Texas at Austin, 100 E. 24th St., Stop A1590, Austin, TX 78712-1224, USA.
Nanomaterials (Basel). 2022 Jun 30;12(13):2254. doi: 10.3390/nano12132254.
We report on the use of silver nanodisks (AgNDs), having a diameter of 50 ± 8 nm and a thickness of 8 ± 2 nm, as electrochemical labels for the detection of a model metalloimmunoassay for the heart failure biomarker NT-proBNP. The detection method is based on an electrochemically activated galvanic exchange (GE) followed by the detection of Ag using anodic stripping voltammetry (ASV). The AgNDs labels are superior to Ag nanocubes and Ag nanospheres in terms of the dynamic range for both the model and NT-proBNP metalloimmunoassays. The linear dynamic range for the model composite is 1.5 to 30.0 pM AgNDs. When AgND labels are used for the NT-proBNP assay, the dynamic range is 0.03-4.0 nM NT-proBNP. The latter range fully overlaps the risk stratification range for heart failure from 53 pM to 590 pM. The performance improvement of the AgNDs is a result of the specific GE mechanism for nanodisks. Specifically, GE is complete across the face of the AgNDs, leaving behind an incompletely exchanged ring structure composed of both Ag and Au.
我们报道了使用直径为50±8纳米、厚度为8±2纳米的银纳米盘（AgNDs）作为电化学标记物，用于检测心力衰竭生物标志物N末端B型利钠肽原（NT-proBNP）的模型金属免疫分析。该检测方法基于电化学激活的电偶置换（GE），随后使用阳极溶出伏安法（ASV）检测银。在模型和NT-proBNP金属免疫分析的动态范围方面，AgNDs标记物优于银纳米立方体和银纳米球。模型复合物的线性动态范围为1.5至30.0皮摩尔AgNDs。当AgND标记物用于NT-proBNP分析时，动态范围为0.03至4.0纳摩尔NT-proBNP。后一范围与心力衰竭的风险分层范围（53皮摩尔至590皮摩尔）完全重叠。AgNDs的性能提升是纳米盘特定GE机制的结果。具体而言，GE在AgNDs的整个表面上完成，留下由银和金组成的未完全交换的环形结构。
