基于线性和方波溶出伏安法的纳米银标记物检测心力衰竭标志物的效率。
Detection Efficiency of Ag Nanoparticle Labels for a Heart Failure Marker Using Linear and Square-Wave Anodic Stripping Voltammetry.
机构信息
Department of Chemistry, The University of Texas at Austin, 105 E. 24th Street, Stop A5300, Austin, TX 78712-1224, USA.
出版信息
Biosensors (Basel). 2022 Mar 29;12(4):203. doi: 10.3390/bios12040203.
In this article, we compare linear sweep anodic stripping voltammetry (LASV) and square-wave anodic stripping voltammetry (SWASV) for detection of a nano metalloimmunoassay. Two separate immunoassays were examined: a model assay, based on interactions between antibodies, and a sandwich assay for the heart failure marker NT-proBNP. In both cases, one antibody is linked to a magnetic microbead, and one is linked to a spherical Ag nanoparticle label. Electrochemical detection is carried out on a paper device. The three analytical figures of merit studied were the precision of the measurements, the calibration sensitivity, and the limit of detection (LOD). For the NT-proBNP assay, the results show that after optimization of the pulse amplitude and frequency of the potential input for SWASV, the detection efficiency is substantially higher compared to LASV. Specifically, the calibration sensitivity increased by up to ~40 fold, the average coefficient of variation decreased by ~40%, and the (LOD) decreased to 300.0 pM. Finally, for a model immunoassay, a ~10-fold decrease in the LOD was observed for SWASV compared to LASV.
在本文中，我们比较了线性扫描阳极溶出伏安法（LASV）和方波阳极溶出伏安法（SWASV）在纳米金属免疫分析检测中的应用。我们研究了两种不同的免疫分析方法：一种是基于抗体相互作用的模型分析，另一种是心力衰竭标志物 NT-proBNP 的夹心分析。在这两种情况下，一种抗体与磁性微球相连，另一种抗体与球形 Ag 纳米粒子标签相连。电化学检测在纸质装置上进行。我们研究了三个分析关键指标，分别是测量精度、校准灵敏度和检测限（LOD）。对于 NT-proBNP 分析，结果表明，通过优化 SWASV 的电位输入脉冲幅度和频率，与 LASV 相比，检测效率显著提高。具体而言，校准灵敏度提高了约 40 倍，平均变异系数降低了约 40%，检测限（LOD）降低至 300.0 pM。最后，对于模型免疫分析，与 LASV 相比，SWASV 的 LOD 降低了约 10 倍。
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