用于电化学检测 Ag 纳米粒子标记物的混合纸和 3D 打印微流控装置。
Hybrid paper and 3D-printed microfluidic device for electrochemical detection of Ag nanoparticle labels.
机构信息
Department of Chemistry and Texas Materials Institute, The University of Texas at Austin, 100 East 24th Street, Stop A1590, Austin, Texas 78712-1224, USA.
Interactives Executive Excellence LLC, Austin, Texas 78733, USA.
出版信息
Lab Chip. 2020 May 5;20(9):1648-1657. doi: 10.1039/d0lc00276c.
In the present article we report a new hybrid microfluidic device (hyFlow) comprising a disposable paper electrode and a three-dimensional (3D) printed plastic chip for the electrochemical detection of a magnetic bead-silver nanoparticle (MB-AgNP) bioconjugate. This hybrid device evolved due to the difficulty of incorporating micron-scale MBs into paper-only fluidic devices. Specifically, paper fluidic devices can entrap MB-containing conjugates within their cellulose or nitrocellulose fiber matrix. The hyFlow system was designed to minimize such issues and transport MB conjugates more efficiently to the electrochemical detection zone of the device. The hyFlow system retains the benefit of fluid transport by pressure-driven flow, however, no pump is required for its operation. The hyFlow device is capable of detecting either pre-formed MB-AgNP conjugates or conjugates formed in situ. The detection limit of AgNPs using this device is 12 pM, which represents just 22 AgNPs per MB.
在本文中，我们报告了一种新的混合微流控装置（hyFlow），该装置由一次性纸电极和三维（3D）打印塑料芯片组成，用于电化学检测磁珠-银纳米粒子（MB-AgNP）生物缀合物。这种混合装置的出现是由于将微米级 MB 纳入仅用纸制成的微流控装置存在困难。具体来说，纸基微流控装置可以将含有 MB 的缀合物困在纤维素或硝酸纤维素纤维基质内。hyFlow 系统旨在最小化这些问题，并更有效地将 MB 缀合物输送到装置的电化学检测区域。hyFlow 系统保留了压力驱动流进行流体输送的优势，但无需泵即可运行。hyFlow 装置能够检测预先形成的 MB-AgNP 缀合物或原位形成的缀合物。使用该装置检测 AgNP 的检测限为 12 pM，这代表每个 MB 只有 22 个 AgNP。
Zotero 插件