Taylor D E, Whamond J S, Penhallow J E
Br J Surg. 1987 Apr;74(4):316-9. doi: 10.1002/bjs.1800740430.
The impairment of healing in laparotomy wounds in rats with compensated oligaemia has been studied in two parallel investigations. Animals bled 1 ml/100 g body weight (bled) were compared with unbled animals undergoing the same operation (control). Firstly, wounds were assessed after 22 days for strength of skin and muscle layers (11 control: 9 bled). Both layers were significantly weaker in bled animals; by 36 per cent for skin (P less than 0.02) and by 22 per cent for muscle (P = 0.02). Secondly, wound fibroblast function was assessed after 10 days by quantitative histology and autoradiography (30 control: 31 bled). There were no significant differences between the two groups for fibroblast density, replication ([3H]thymidine), or general protein synthesis ([3H]leucine). Bled animals had less dense collagen packing by 21 per cent (P less than 0.01), but 33 per cent more incorporation of ([3H]proline) by fibroblasts (P less than 0.01) compared with control animals. The effect of compensated oligaemia on wound healing is more marked in skin than in muscle; it appears to be a specific increase in collagen turnover with the increase in reabsorption exceeding that in synthesis.
在两项平行研究中，对代偿性低血容量大鼠剖腹手术伤口愈合受损情况进行了研究。将体重每100克失血1毫升的动物（失血组）与接受相同手术的未失血动物（对照组）进行比较。首先，在22天后评估伤口皮肤和肌肉层的强度（11只对照组：9只失血组）。失血组动物的两层强度均显著较弱；皮肤层弱36%（P<0.02），肌肉层弱22%（P = 0.02）。其次，在10天后通过定量组织学和放射自显影评估伤口成纤维细胞功能（30只对照组：31只失血组）。两组在成纤维细胞密度、复制（[3H]胸腺嘧啶核苷）或一般蛋白质合成（[3H]亮氨酸）方面无显著差异。与对照组动物相比，失血组动物的胶原纤维堆积密度低21%（P<0.01），但成纤维细胞对（[3H]脯氨酸）的掺入量高33%（P<0.01）。代偿性低血容量对伤口愈合的影响在皮肤中比在肌肉中更明显；似乎是胶原周转特异性增加，重吸收增加超过合成增加。
