Gorodetsky R, McBride W H, Withers H R, Miller G G
UCLA Medical Center 90024-1714.
Radiat Res. 1991 Feb;125(2):181-6.
The role of dermal fibroblasts in the expression of radiation-induced damage to the skin was studied. Fibroblasts from neonatal mice were cultured, harvested, and injected into full-depth surgical incisions in the dorsal area of mouse skin, which had been previously locally irradiated by 18 Gy X rays. As a control, cells irradiated with a dose of 20 Gy were also injected. The effect of radiation and fibroblast implants on the gain of skin wound strength was assayed. In an additional experiment freshly isolated cells were implanted. Two weeks following wounding the irradiated skin had reached only about a third of the strength of unirradiated skin. A significant increase of wound strength in irradiated skin was observed when 1.5-2 x 10(6) cultured fibroblasts or freshly isolated fibroblasts were injected into the 20-mm-long wound bed. Irradiated cells had significantly less effect. This suggests that implanting isolated syngeneic cells may "rescue" wounds from the effect of prior irradiation. Semiquantitative immunohistology of types I and III collagen was performed in parallel using a video image digitizing system. Levels of both types I and III collagen were altered in the dermis and the wound tissues in irradiated skin, but the implant of cultured fibroblasts did not affect notably the total levels and the disposition of the two collagen isotypes.
研究了真皮成纤维细胞在皮肤辐射损伤表达中的作用。从小鼠新生儿中分离出成纤维细胞，进行培养、收获，然后注射到小鼠背部皮肤的全层手术切口中，该部位先前已接受18 Gy X射线局部照射。作为对照，还注射了剂量为20 Gy照射过的细胞。测定了辐射和成纤维细胞植入对皮肤伤口强度增加的影响。在另一项实验中植入了新鲜分离的细胞。受伤两周后，受照射皮肤的强度仅达到未受照射皮肤强度的约三分之一。当将1.5 - 2×10⁶个培养的成纤维细胞或新鲜分离的成纤维细胞注射到20毫米长的伤口床中时，观察到受照射皮肤的伤口强度有显著增加。照射过的细胞效果明显较差。这表明植入分离的同基因细胞可能会使伤口从先前照射的影响中“恢复”。使用视频图像数字化系统同时进行了I型和III型胶原的半定量免疫组织学分析。受照射皮肤的真皮和伤口组织中I型和III型胶原的水平均发生了改变，但培养的成纤维细胞植入对这两种胶原同型的总水平和分布没有明显影响。
