College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen 518000, China.
Sensors (Basel). 2022 Jun 24;22(13):4793. doi: 10.3390/s22134793.
Blood coagulation is a complicated dynamic process that maintains the blood's fluid state and prevents uncontrollable bleeding. The real-time monitoring of coagulation dynamics is critical for blood transfusion guidance, emergency management of trauma-induced coagulopathy, perioperative bleeding, and targeted hemostatic therapy. Here, we utilize optical vortex dynamics to detect the blood coagulation dynamic process in a rapid and non-contact manner. To characterize the temporal changes in viscoelastic properties of blood during coagulation, we track the stochastic motion of optical vortices in the time-varying speckles reflected from 100 blood samples with varied coagulation profiles. The mean square displacement (MSD) of the vortices increases nonlinearly with time lag during blood coagulation reminiscent of the particles in viscoelastic fluids. The MSD curves with coagulation time are similar to the tracings of thromboelastography (TEG) during the blood coagulation. The retrieved coagulation parameters, such as reaction time and activated clotting time measured using the optical vortex method, exhibit a close correlation to those parameters acquired from TEG. These results demonstrate the feasibility of the optical vortex method for monitoring blood coagulation at the point of care. Our method is also applicable to measuring the viscoelasticity of complex fluids and turbid soft matters.
血液凝固是一个复杂的动态过程，它维持着血液的液态状态，并防止不可控的出血。实时监测凝血动力学对于输血指导、创伤性凝血病的紧急管理、围手术期出血和靶向止血治疗至关重要。在这里，我们利用光学涡旋动力学来快速、非接触式地检测血液凝固的动态过程。为了描述血液在凝固过程中粘弹性的时间变化，我们跟踪了 100 个具有不同凝固特征的血液样本中，由时变散斑反射的光学涡旋的随机运动。在血液凝固过程中，涡旋的均方位移（MSD）随时间滞后呈非线性增加，类似于粘弹性流体中的粒子。MSD 曲线与凝血时间的轨迹与血栓弹性描记图（TEG）在血液凝固过程中的轨迹相似。使用光学涡旋方法测量的反应时间和激活凝血时间等凝血参数与 TEG 获得的参数密切相关。这些结果表明，光学涡旋方法在即时护理点监测血液凝固是可行的。我们的方法也适用于测量复杂流体和浑浊软物质的粘弹性。
