急性缺血性脑卒中患者 JNK 通路相关磷酸酶：与辅助性 T 细胞、临床特征及复发风险的相关性。
JNK pathway-associated phosphatase in acute ischemic stroke patients: Its correlation with T helper cells, clinical properties, and recurrence risk.
机构信息
Second Department of Neurology, HanDan Central Hospital, Handan, China.
First Department of Pediatric Surgery, HanDan Central Hospital, Handan, China.
出版信息
J Clin Lab Anal. 2022 Aug;36(8):e24535. doi: 10.1002/jcla.24535. Epub 2022 Jul 8.
OBJECTIVE
JKAP modifies T-cell immune response and inflammation, also involves in cardia-cerebrovascular disease etiology. This study intended to explore JKAP's relation with T-helper 1 (Th1), T-helper 17 (Th17) cell levels, clinical properties, and recurrence-free survival (RFS) in acute ischemic stroke (AIS) patients.
METHODS
A total of 155 AIS patients were analyzed. Serum JKAP, interferon-gamma (IFN-γ), and interleukin-17A (IL-17A) were detected by ELISA; then blood Th1 and Th17 cells were quantified by flow cytometry. Besides, 30 healthy subjects were enrolled as controls to detect JKAP, Th1, and Th17 cells.
RESULTS
JKAP level was lower (p < 0.001), Th1 cells were not differed (p = 0.068), but Th17 cells were elevated in AIS patients versus controls (p < 0.001). Meanwhile, JKAP was negatively correlated with Th1 cells (p = 0.038), Th17 cells (P<0.001), IFN-γ (p = 0.002), and IL-17A (p < 0.001) in AIS patients. JKAP was negatively associated with the National Institutes of Health Stroke Scale (NIHSS) score (p < 0.001), but Th17 cells (p = 0.001), IFN-γ (p = 0.035), and IL-17A (p = 0.008) levels were positively associated with NIHSS score. Additionally, accumulating RFS was numerically longer in patients with JKAP Quantile (Q) 4 than patients with JKAP Q1-Q3 (p = 0.068), and numerically better in patients with JKAP Q3-Q4 than patients with JKAP Q1-Q2 (p = 0.069), but without statistical significance.
CONCLUSION
JKAP correlates with lower Th1 and Th17 cell percentages as well as milder disease severity.
目的
JKAP 可调节 T 细胞免疫应答和炎症反应，并且与心血脑血管疾病的病因有关。本研究旨在探讨 JKAP 与辅助性 T 细胞 1（Th1）、辅助性 T 细胞 17（Th17）细胞水平、临床特征和急性缺血性脑卒中（AIS）患者无复发生存率（RFS）之间的关系。
方法
共分析了 155 例 AIS 患者。采用酶联免疫吸附试验（ELISA）检测血清 JKAP、干扰素-γ（IFN-γ）和白细胞介素-17A（IL-17A）；采用流式细胞术定量检测血 Th1 和 Th17 细胞。此外，还招募了 30 名健康受试者作为对照，以检测 JKAP、Th1 和 Th17 细胞。
结果
与对照组相比，AIS 患者的 JKAP 水平较低（p<0.001），Th1 细胞无差异（p=0.068），但 Th17 细胞升高（p<0.001）。同时，JKAP 与 AIS 患者的 Th1 细胞（p=0.038）、Th17 细胞（P<0.001）、IFN-γ（p=0.002）和 IL-17A（p<0.001）呈负相关。JKAP 与美国国立卫生研究院卒中量表（NIHSS）评分呈负相关（p<0.001），但 Th17 细胞（p=0.001）、IFN-γ（p=0.035）和 IL-17A（p=0.008）水平与 NIHSS 评分呈正相关。此外，JKAP 四分位数（Q）4 的患者的累积 RFS 数值上长于 JKAP Q1-Q3 的患者（p=0.068），JKAP Q3-Q4 的患者的累积 RFS 数值上长于 JKAP Q1-Q2 的患者（p=0.069），但无统计学意义。
结论
JKAP 与较低的 Th1 和 Th17 细胞百分比以及较轻的疾病严重程度相关。
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