Han Dong Hoon, Lee Min Jeon, Kim Jeong Hee
Department of Biochemistry, College of Dentistry, Kyung Hee University, Seoul 130-701, Korea.
Anticancer Res. 2006 Sep-Oct;26(5A):3601-6.
Antioxidant, antiproliferative and apoptosis inducing activities of a natural polyphenolic compound, ellagic acid, were studied.
DPPH radical scavenging and lipid peroxidation inhibitory activities were observed. Activities of antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) were measured in ellagic acid-treated V79-4 cells. For apoptotic inducing activity, human osteogenic sarcoma (HOS) cell proliferation, chromosomal DNA degradation and changes in apoptosis-related protein levels were measured.
Ellagic acid showed high DPPH radical scavenging and lipid peroxidation inhibition activities. SOD, CAT and GPX activities were significantly increased in ellagic acid-treated V79-4 cells. Ellagic acid significantly reduced HOS cell proliferation, and induced apoptosis evidenced by chromosomal DNA degradation and apoptotic body appearance. Bax expression was induced and caspase-3 was activated by ellagic acid treatment.
Ellagic acid exhibited both antioxidant activity in V79-4 cells and apoptosis-inducing activity in HOS cells through the up-regulation of Bax and activation of caspase-3.
研究了天然多酚化合物鞣花酸的抗氧化、抗增殖和诱导凋亡活性。
观察了二苯基苦味酰基自由基清除和脂质过氧化抑制活性。在经鞣花酸处理的V79 - 4细胞中测定了抗氧化酶超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPX）的活性。对于诱导凋亡活性，测定了人骨肉瘤（HOS）细胞增殖、染色体DNA降解以及凋亡相关蛋白水平的变化。
鞣花酸表现出较高的二苯基苦味酰基自由基清除和脂质过氧化抑制活性。在经鞣花酸处理的V79 - 4细胞中，SOD、CAT和GPX活性显著增加。鞣花酸显著降低了HOS细胞增殖，并通过染色体DNA降解和凋亡小体出现证明诱导了凋亡。鞣花酸处理诱导了Bax表达并激活了caspase - 3。
鞣花酸通过上调Bax和激活caspase - 3，在V79 - 4细胞中表现出抗氧化活性，在HOS细胞中表现出诱导凋亡活性。
