State Key Laboratory of Hybrid Rice and Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha, China.
Long Ping Branch, Graduate School of Hunan University, Changsha, China.
Front Cell Infect Microbiol. 2022 Jun 24;12:926771. doi: 10.3389/fcimb.2022.926771. eCollection 2022.
Glycosylphosphatidylinositol (GPI) anchoring the protein GPI modification post-transcriptionally is commonly seen. In our previous study, MoPer1, a GPI anchoring essential factor, has a critical effect on growth, pathogenicity, and conidiogenesis, but its molecular mechanism is not clear. Here, we extracted the glycoproteins from the Δ mutant and wild-type Guy11 to analyze their differential levels by quantitative proteomic analysis of TMT markers. After background subtraction, a total of 431 proteins, with significant changes in expression, were successfully identified, and these differential proteins were involved in biological regulation, as well as cellular process and metabolic process, binding, catalytic activity, and other aspects. Moreover, we found that MoPer1 regulates the expression of 14 proteins involved in growth, development, and pathogenicity of . The above findings shed light on MoPer1's underlying mechanism in regulating growth, development, and pathogenicity of .
糖基磷脂酰肌醇（GPI）锚定蛋白的 GPI 修饰是常见的转录后修饰。在我们之前的研究中，GPI 锚定必需因子 MoPer1 对 生长、致病性和分生孢子形成有重要影响，但它的分子机制尚不清楚。在这里，我们从Δ突变体和野生型 Guy11 中提取糖蛋白，通过 TMT 标记的定量蛋白质组学分析来分析它们的差异水平。扣除背景后，成功鉴定出 431 个表达有显著变化的蛋白质，这些差异蛋白参与了生物调节、细胞过程和代谢过程、结合、催化活性等方面。此外，我们发现 MoPer1 调节 14 个与生长、发育和致病性相关蛋白的表达。上述发现为 MoPer1 调节 生长、发育和致病性的潜在机制提供了线索。
