苯甲羟肟酸诱导海胆（刺冠海胆）发生多精受精。

Benzohydroxamic acid induces polyspermic fertilization in the sea urchin Arbacia punctulata.

作者信息

Schuel H, Schuel R

出版信息

Cell Biol Int Rep. 1987 Mar;11(3):189-96. doi: 10.1016/0309-1651(87)90025-7.

Abstract

Benzohydroxamic acid (BHA) is a competitive inhibitor of the sea urchin sperm peroxidase. We now report that addition of BHA to fertilization cultures of Arbacia punctulata promotes polyspermy. This effect is dose and sperm density dependent. The cortical reaction (elevation of the fertilization envelope) is not retarded by BHA. BHA must be added to the cultures before the eggs complete the cortical reaction at 60 sec post insemination in order to induce polyspermy. Since sea urchin eggs release H2O2 during the cortical reaction at fertilization, these findings support our hypothesis that the sperm peroxidase has a functional role in helping to prevent polyspermy.

摘要

苯甲羟肟酸（BHA）是海胆精子过氧化物酶的竞争性抑制剂。我们现在报告，在斑点海胆受精培养物中添加BHA会促进多精受精。这种效应取决于剂量和精子密度。皮层反应（受精膜升高）不受BHA的抑制。必须在授精后60秒卵子完成皮层反应之前将BHA添加到培养物中，以诱导多精受精。由于海胆卵在受精时的皮层反应过程中会释放过氧化氢，这些发现支持了我们的假设，即精子过氧化物酶在帮助防止多精受精方面具有功能性作用。