Li Yang, Liu Bingjun, Chen Jian, Yue Xuelian
State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines, Anhui University of Science & Technology, Huainan, Anhui 232001, China.
Institute of Energy, Hefei Comprehensive National Science Center, Hefei, Anhui 230031, China.
ACS Omega. 2022 Jun 22;7(26):22671-22681. doi: 10.1021/acsomega.2c02126. eCollection 2022 Jul 5.
Coal microbes are the predominant form of life in the subsurface ecosystem, which play a vital role in biogeochemical cycles. However, the systematic information about carbon-nitrogen-sulfur (C-N-S)-related microbial communities in coal seams is limited. In this study, 16S rRNA gene data from a total of 93 microbial communities in coals were collected for meta-analysis. The results showed that 718 functional genera were related to the C-N-S cycle, wherein N fixation, denitrification, and C degradation groups dominated in relative abundance, Chao1 richness, Shannon diversity, and niche width. Genus having the most C-N-S-related functions showed the highest relative abundance, and genus with a higher abundance participated in C degradation, CH oxidation, N fixation, ammoxidation, and denitrification. Such was a core genus in the co-occurrence network of microbial prokaryotes and showed higher levels in weight degree, betweenness centrality, and eigenvector centrality. In addition, most of the methanogens could fix N and dominated in the N fixation groups. Among them, genera and showed higher levels in the betweenness centrality index. In addition, the genus was linked to the methanogenesis co-occurrence network module. In parallel, the S reduction gene was present in the highest total relative abundance of genes, followed by the C degradation and the denitrification genes, and S genes (especially genes) were the main genes linked to the co-occurrence network of the C-N-S-related genes. In summary, this study strengthened our knowledge regarding the C-N-S-related coal microbial communities, which is of great significance in understanding the microbial ecology and geochemical cycle of coals.
煤微生物是地下生态系统中主要的生命形式，在生物地球化学循环中发挥着至关重要的作用。然而，关于煤层中与碳 - 氮 - 硫（C - N - S）相关的微生物群落的系统信息有限。在本研究中，收集了来自93个煤中微生物群落的16S rRNA基因数据进行荟萃分析。结果表明，有718个功能属与C - N - S循环相关，其中固氮、反硝化和碳降解组在相对丰度、Chao1丰富度、香农多样性和生态位宽度方面占主导地位。具有最多C - N - S相关功能的属显示出最高的相对丰度，丰度较高的属参与碳降解、CH氧化、固氮、氨氧化和反硝化。这样的属是微生物原核生物共现网络中的核心属，在权重度、介数中心性和特征向量中心性方面表现较高。此外，大多数产甲烷菌能够固氮并在固氮组中占主导地位。其中，属 和 在介数中心性指数方面表现较高。此外，属 与产甲烷作用共现网络模块相关联。同时，硫还原基因在基因总相对丰度中最高，其次是碳降解和反硝化基因，并且硫基因（特别是 基因）是与C - N - S相关基因共现网络相关的主要基因。总之，本研究加强了我们对与C - N - S相关的煤微生物群落的认识，这对于理解煤的微生物生态学和地球化学循环具有重要意义。
