School of Safety Science and Engineering, Anhui University of Science and Technology, Huainan, China.
Joint National-Local Engineering Research Centre for Safe and Precise Coal Mining, Anhui University of Science and Technology, Huainan, China.
Pol J Microbiol. 2022 Sep 24;71(3):359-370. doi: 10.33073/pjm-2022-032. eCollection 2022 Sep 1.
Nutrient stimulation is considered effective for improving biogenic coalbed methane production potential. However, our knowledge of the microbial assembly process for profuse and rare microbial communities in coals under nutrient stimulation is still limited. This study collected 16S rRNA gene data from 59 microbial communities in coals for a meta-analysis. Among these communities, 116 genera were identified as profuse taxa, and the remaining 1,637 genera were identified as rare taxa. Nutrient stimulation increased the Chao1 richness of profuse and rare genera and changed the compositions of profuse and rare genera based on nonmetric multidimensional scaling with Bray-Curtis dissimilarities. In addition, many profuse and rare genera belonging to Proteobacteria and Acidobacteria were reduced, whereas those belonging to Euryarchaeota and Firmicutes were increased under nutrient stimulation. Concomitantly, the microbial co-occurrence relationship network was also altered by nutrient addition, and many rare genera mainly belonging to Firmicutes, , and Euryarchaeota also comprised the key microorganisms. In addition, the compositions of most of the profuse and rare genera in communities were driven by stochastic processes, and nutrient stimulation increased the relative contribution of dispersal limitation for both profuse and rare microbial community assemblages and that of variable selection for rare microbial community assemblages. In summary, this study strengthened our knowledge regarding the mechanistic responses of coal microbial diversity and community composition to nutrient stimulation, which are of great importance for understanding the microbial ecology of coals and the sustainability of methane production stimulated by nutrients.
营养刺激被认为可以有效地提高生物成因煤层甲烷的生产潜力。然而，我们对于营养刺激下煤中丰富和稀有微生物群落的微生物组装过程的认识仍然有限。本研究通过元分析从 59 个煤微生物群落中收集了 16S rRNA 基因数据。在这些群落中，鉴定出 116 个属为丰富类群，其余 1637 个属为稀有类群。营养刺激增加了丰富和稀有属的 Chao1 丰富度，并基于非度量多维尺度分析和 Bray-Curtis 不相似性改变了丰富和稀有属的组成。此外，许多属于变形菌门和酸杆菌门的丰富和稀有属减少，而属于广古菌门和厚壁菌门的属增加。同时，营养添加也改变了微生物共生关系网络，许多主要属于厚壁菌门、放线菌门和广古菌门的稀有属也构成了关键微生物。此外，群落中大多数丰富和稀有属的组成主要由随机过程驱动，营养刺激增加了丰富和稀有微生物群落组装的扩散限制的相对贡献，以及稀有微生物群落组装的可变选择的相对贡献。总之，本研究加强了我们对营养刺激下煤微生物多样性和群落组成的机制响应的认识，这对于理解煤的微生物生态学和营养刺激下甲烷生产的可持续性具有重要意义。
