验证（鸡矢藤）在正常健康 Sprague-Dawley 大鼠中的降血糖和降血压作用。

Validating the Hypoglycaemic and Hypotensive Roles of (Chicken Weed) in Normal Healthy Sprague-Dawley Rats.

机构信息

Department of Basic Medical Sciences, Faculty of Medical Sciences, The University of the West Indies, Kingston 7, Jamaica.

Department of Chemistry, Faculty of Science & Technology, The University of the West Indies, Kingston 7, Jamaica.

出版信息

ScientificWorldJournal. 2022 Jun 29;2022:6547734. doi: 10.1155/2022/6547734. eCollection 2022.

DOI:10.1155/2022/6547734

PMID:35812002

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9259359/

Abstract

Diabetes mellitus (DM) is an endocrine disease and is characterized by hyperglycaemia. L. (chicken weed) has been used in traditional medicine to treat various ailments including DM. Aqueous, hexane, ethyl acetate, and methanol crude extracts of L. were investigated for their anti-oxidant activities and hypoglycaemic and hypotensive effects in normal, healthy Sprague-Dawley rats using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, oral glucose tolerance test (OGTT), and the CODA noninvasive blood pressure system to determine systolic blood pressure (SBP), diastolic blood pressure (DBP), mean arterial pressure (MAP), and heart rate (HR). The aqueous extract caused a free radical scavenging effect with an IC value of 10.2 ± 1.01 g/mL versus vitamin C (9.42 ± 1.01 g/mL). The extract lowered the blood glucose concentration at the 150 minute interval (5.00 ± 0.22 mM vs. 6.51 ± 0.33 mM; = 0.004) and the 180 minute interval (4.77 ± 0.27 mM vs. 5.93 ± 0.0.30 mM; = 0.015). The hexane extract gave significant hypoglycaemic activity at the 120 minute interval (4.54 ± 0.21 mmol/L vs. 5.50 ± 0.17 mmol/L; = 0.005). The hexane extract also significantly lowered the SBP (132 ± 6 mm Hg; = 0.014), DBP (106 ± 7 mm Hg; = 0.034), and MAP (114 ± 7 mm Hg; = 0.023) versus the controls SBP (156 ± 4 mm Hg), DBP (132 ± 8 mm Hg), and MAP (140 ± 6 mm Hg). Bioassay-directed purification of the hexane extract yielded 3,7,11-trimethyl-1,6,10-dodecatrien-3-ol (1), 3,7,11-trimethyl-2,6,10-dodecatrien-1-ol (2), and 5,22-stigmastadien-3-ol (3) as active principles. Hence, L. showed anti-oxidant, hypoglycaemic, and hypotensive effects in the rats and may have potential applications in the treatment of diabetes.

摘要

糖尿病（DM）是一种内分泌疾病，其特征是高血糖。鸡矢藤已被用于传统医学中治疗各种疾病，包括 DM。使用 2,2-二苯基-1-苦基肼（DPPH）测定法、口服葡萄糖耐量试验（OGTT）和 CODA 无创血压系统，研究了鸡矢藤的水提物、正己烷提物、乙酸乙酯提物和甲醇提物的抗氧化活性以及对正常健康 Sprague-Dawley 大鼠的降血糖和降压作用，以测定收缩压（SBP）、舒张压（DBP）、平均动脉压（MAP）和心率（HR）。水提物具有自由基清除作用，IC 值为 10.2 ± 1.01 g/mL，与维生素 C（9.42 ± 1.01 g/mL）相当。该提取物可降低 150 分钟间隔时的血糖浓度（5.00 ± 0.22 mM 对 6.51 ± 0.33 mM； = 0.004）和 180 分钟间隔时的血糖浓度（4.77 ± 0.27 mM 对 5.93 ± 0.0.30 mM； = 0.015）。正己烷提取物在 120 分钟间隔时具有显著的降血糖活性（4.54 ± 0.21 mmol/L 对 5.50 ± 0.17 mmol/L； = 0.005）。正己烷提取物还显著降低了 SBP（132 ± 6 mmHg； = 0.014）、DBP（106 ± 7 mmHg； = 0.034）和 MAP（114 ± 7 mmHg； = 0.023），与对照组 SBP（156 ± 4 mmHg）、DBP（132 ± 8 mmHg）和 MAP（140 ± 6 mmHg）相比。正己烷提取物的生物测定导向纯化得到了 3,7,11-三甲基-1,6,10-十二碳三烯-3-醇（1）、3,7,11-三甲基-2,6,10-十二碳三烯-1-醇（2）和 5,22-豆甾二烯-3-醇（3），这些物质为活性成分。因此，鸡矢藤在大鼠中表现出抗氧化、降血糖和降压作用，可能具有治疗糖尿病的潜在应用价值。