Kandwal Prashant, Fujiwara Toru, Kamiya Takehiro
Laboratory of Plant Nutrition and Fertilizers, Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Sciences, The University of Tokyo, Tokyo, Japan.
Front Plant Sci. 2022 Jun 22;13:868661. doi: 10.3389/fpls.2022.868661. eCollection 2022.
Nearly 2 billion people who reside in developing countries are suffering from nutrient deficiency, also known as hidden hunger. A hidden hunger includes iron (Fe) and zinc (Zn) deficiency. One of the most efficient solutions to hidden hunger is the biofortification of crops through breeding. In this study, we characterized the mutant 1095_k, which has high grain Fe (1.4-fold) and Zn (1.2-fold) concentration compared with wild-type plants for a 5-year field trial. The yield components of 1095_k are similar to wild-type plants in a paddy field. In addition, 1095_k has a non-sense mutation in , a vacuolar localized Fe transporter. F2 crosses between 1095_k and wild type having the mutation showing higher grain Fe and Zn concentration. In contrast, plants without the mutation showed similar element concentrations as the wild type. These results suggest that would be responsible for high Fe and Zn of grain and the 1095_k would be a useful breeding material for the biofortification of Fe and Zn.
近20亿生活在发展中国家的人正遭受营养缺乏,也被称为隐性饥饿。隐性饥饿包括铁(Fe)和锌(Zn)缺乏。解决隐性饥饿最有效的方法之一是通过育种对作物进行生物强化。在本研究中,我们对突变体1095_k进行了表征,在为期5年的田间试验中,与野生型植物相比,其籽粒铁含量(约1.4倍)和锌含量(约1.2倍)较高。在稻田中,1095_k的产量构成因素与野生型植物相似。此外,1095_k在一种液泡定位的铁转运蛋白中存在无义突变。1095_k与具有该突变的野生型之间的F2杂交后代籽粒中铁和锌浓度较高。相比之下,没有该突变的植株元素浓度与野生型相似。这些结果表明,该基因会导致籽粒中铁和锌含量较高,而1095_k将是铁和锌生物强化的有用育种材料。