Shi Yan, Qin Yuan, Li Fenglan, Wang Haifeng
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
College of Life Science, Northeast Agricultural University, Harbin, China.
Front Plant Sci. 2022 Jun 23;13:934379. doi: 10.3389/fpls.2022.934379. eCollection 2022.
Potato is an important food crop that occupies lesser area but has greater production than rice and wheat. However, potato production is affected by numerous biotic and abiotic stresses, among which dry rot is a disease that has significant effect on potato production, storage, and processing. However, the role of DNA methylation in regulating potato response to toxin deoxynivalenol (DON) stress is still not fully understood. In this study, we performed DNA methylome and transcriptome analyses of potato tubers treated with five concentrations of DON. The global DNA methylation levels in potato tubers treated with different concentrations of DON showed significant changes relative to those in the control. In particular, the 20 ng/ml treatment showed the largest decrease in all three contexts of methylation levels, especially CHH contexts in transposon regions. The differentially methylated region (DMR)-associated differentially expressed genes (DEGs) were significantly enriched in resistance-related metabolic pathways, indicating that DNA methylation plays an essential role in potato response to DON stress. Furthermore, we examined lesions on potato tubers infested with after treatment. Furthermore, the potato tubers treated with 5 and 35 ng/ml DON had lesions of significantly smaller diameters than those of the control, indicating that DON stress may induce resistance. We speculate that this may be related to epigenetic memory created after DNA methylation changes. The detailed DNA methylome and transcriptome profiles suggest that DNA methylation plays a vital role in potato disease resistance and has great potential for enhancing potato dry rot resistance.
马铃薯是一种重要的粮食作物，种植面积比水稻和小麦小，但产量却高于它们。然而，马铃薯生产受到多种生物和非生物胁迫的影响，其中干腐病是一种对马铃薯生产、储存和加工有重大影响的病害。然而，DNA甲基化在调节马铃薯对毒素脱氧雪腐镰刀菌烯醇（DON）胁迫反应中的作用仍未完全了解。在本研究中，我们对用五种浓度的DON处理的马铃薯块茎进行了DNA甲基化组和转录组分析。与对照相比，用不同浓度的DON处理的马铃薯块茎中的全局DNA甲基化水平显示出显著变化。特别是，20 ng/ml处理在所有三种甲基化水平背景下均显示出最大程度的下降，尤其是转座子区域中的CHH背景。差异甲基化区域（DMR）相关的差异表达基因（DEG）在抗性相关代谢途径中显著富集，表明DNA甲基化在马铃薯对DON胁迫的反应中起重要作用。此外，我们检查了处理后感染马铃薯块茎的病斑。此外，用5和35 ng/ml DON处理的马铃薯块茎的病斑直径明显小于对照，表明DON胁迫可能诱导抗性。我们推测这可能与DNA甲基化变化后产生的表观遗传记忆有关。详细的DNA甲基化组和转录组图谱表明，DNA甲基化在马铃薯抗病性中起关键作用，并且在增强马铃薯抗干腐病方面具有巨大潜力。
