Song Yuan, Jia Zhifeng, Hou Yukang, Ma Xiang, Li Lizhen, Jin Xing, An Lizhe
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, China.
Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, China.
Front Plant Sci. 2020 Dec 7;11:608540. doi: 10.3389/fpls.2020.608540. eCollection 2020.
Plants experience a wide array of environmental stimuli, some of which are frequent occurrences of cold weather, which have priming effects on agricultural production and agronomic traits. DNA methylation may act as an epigenetic regulator for the cold response of Tartary buckwheat (). Combined with long-term field observation and laboratory experiments, comparative phenome, methylome, and transcriptome analyses were performed to investigate the potential epigenetic contributions for the cold priming of Tartary buckwheat variety Dingku1. Tartary buckwheat cv. Dingku1 exhibited low-temperature resistance. Single-base resolution maps of the DNA methylome were generated, and a global loss of DNA methylation was observed during cold responding in Dingku1. These sites with differential methylation levels were predominant in the intergenic regions. Several hundred genes had different DNA methylation patterns and expressions in different cold treatments (cold memory and cold shock), such as , , and . The application of a DNA methylation inhibitor caused a change of the free lysine content, suggesting that DNA methylation can affect metabolite accumulation for Tartary buckwheat cold responses. The results of the present study suggest important roles of DNA methylation in regulating cold response and forming agronomic traits in Tartary buckwheat.
植物会经历各种各样的环境刺激，其中一些是寒冷天气的频繁出现，这对农业生产和农艺性状具有引发效应。DNA甲基化可能作为苦荞对寒冷反应的一种表观遗传调节因子。结合长期田间观察和实验室实验，进行了比较表型组、甲基化组和转录组分析，以研究苦荞品种定苦1号冷引发潜在的表观遗传贡献。苦荞品种定苦1号表现出耐低温性。生成了DNA甲基化组的单碱基分辨率图谱，并且在定苦1号的冷反应过程中观察到DNA甲基化的整体丢失。这些具有不同甲基化水平的位点在基因间区域占主导地位。数百个基因在不同的冷处理（冷记忆和冷激）中具有不同的DNA甲基化模式和表达，例如 、 和 。DNA甲基化抑制剂的应用导致游离赖氨酸含量发生变化，这表明DNA甲基化可以影响苦荞对寒冷反应的代谢物积累。本研究结果表明DNA甲基化在调节苦荞的寒冷反应和形成农艺性状方面具有重要作用。
