Ji Haiwang, Jin Xiang, Zhang Qing, Zhou Yuan, Zhu Chan, Yang Yan, Tang Zongxiang, Yu Guang, Wang Changming
School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Front Behav Neurosci. 2022 Jun 23;16:873750. doi: 10.3389/fnbeh.2022.873750. eCollection 2022.
Cancer induced bone pain (CIBP) occurs in patients with advanced osteosarcoma or metastasized bone tumors that can negatively affects the patient's quality of life. However, motor impairment in CIBP is still understudied. To improve the quality of life of patients with CIBP, the study of CIBP induced movement impairment is of particular importance. Here, we presented a model of metastatic cancer induced bone pain caused by an allograft of Lewis lung cancer cells. In this method, we injected Lewis lung cancer cells into the femoral medulla cavity and recorded the pain behavior and motor behavior after CIBP surgery. We observed enhanced pain after the initial surgery. Interestingly, we found the latency on rotarod was significantly reduced concomitant with tumor growth and pain. This result indicated that the motor coordination and balance were severely impaired in CIBP. We also found the pain and motor behavioral differences in models that severed the patellar ligament vs. maintaining the patellar ligament. These findings provide a novel clue for further investigating the mechanisms responsible for the generation and development of CIBP.
癌症诱发的骨痛（CIBP）发生在患有晚期骨肉瘤或骨转移瘤的患者中，会对患者的生活质量产生负面影响。然而，CIBP中的运动障碍仍未得到充分研究。为了提高CIBP患者的生活质量，研究CIBP诱发的运动障碍尤为重要。在此，我们展示了一种由Lewis肺癌细胞同种异体移植引起的转移性癌症诱发骨痛模型。在这种方法中，我们将Lewis肺癌细胞注入股骨髓腔，并记录CIBP手术后的疼痛行为和运动行为。我们观察到初次手术后疼痛加剧。有趣的是，我们发现随着肿瘤生长和疼痛，转棒试验的潜伏期显著缩短。这一结果表明CIBP中运动协调和平衡严重受损。我们还发现，切断髌韧带与保留髌韧带的模型在疼痛和运动行为上存在差异。这些发现为进一步研究CIBP发生和发展的机制提供了新线索。
