Chu Lijun, Sun Xia, Jia Xiaoju, Li Dazhi, Gao Ping, Zhang Yong, Li Jie
Laboratory of Biological Psychiatry, Institute of Mental Health, Tianjin Anding Hospital, Mental Health Center of Tianjin Medical University, Tianjin, China.
Front Psychiatry. 2022 Jun 23;13:932235. doi: 10.3389/fpsyt.2022.932235. eCollection 2022.
Brain-derived neurotrophic factor (BDNF) is a candidate for susceptibility locus of Panic disorder (PD). However, the findings about the role of the BDNF Val66Met variant in PD were not consistent. Till now, the relationship between BDNF Val66Met polymorphism and anxiety-related traits in PD patients has been rarely explored. This study aimed to explore the relationship among BDNF Val66Met polymorphism, plasma BDNF level and anxiety-related trait in Chinese PD patients.
This multi-center study included 116 PD patients and 99 health controls. We detected single-nucleotide polymorphism (SNP) of BDNF rs6265 (Val66Met) and BDNF plasma level in the two groups. In addition, PD patients were administered the State-Trait Anxiety Inventory (STAI), Panic Disorder Severity Scale-Chinese Version (PDSS-CV) and Hamilton Anxiety Rating Scale (HAMA-14). Quantitative comparison of the differences of BDNF concentration among subjects with different genotypes and association between BDNF Val66Met genotype and trait anxiety were performed.
There were no significant differences in the genotype frequency ( = 0.79) or allele frequency ( = 0.88) between PD patients and health controls. BDNF plasma levels of PD patients were significantly lower than those in control group ( = 0.003). BDNF plasma levels of the Met/Met genotype were significantly lower than those of Val/Met genotype in PD patients ( = 0.033). PD patients carried Met/Met genotype showed significantly higher scores in STAI trait compared to those carried Val/Val genotype ( = 0.045) and Val/Met genotype ( = 0.018). STAI trait scores of PD patients with agoraphobia were significantly higher than those of patients without agoraphobia ( < 0.05). The ANCOVA showed that the dependent variable STAI trait score was significantly affected by factor "genotype" (Val/Val, Val/Met, Met/Met, = 0.029), and covariate "agoraphobia" ( = 0.008). In this model, 11.5% of the variance of the STAI trait score was explained by the BDNF genotype. Contrast analysis showed STAI trait scores of Met/Met subjects were significantly higher than those of Val/Met ( = 0.018) and Val/Val individuals ( = 0.045).
We found that anxiety trait was associated with the BDNF polymorphism in PD patients. BDNF Met/Met genotype may decrease plasma BDNF level and increase trait anxiety in panic disorder.
脑源性神经营养因子（BDNF）是惊恐障碍（PD）易感性位点的一个候选基因。然而，关于BDNF Val66Met变异在PD中的作用的研究结果并不一致。到目前为止，BDNF Val66Met多态性与PD患者焦虑相关特质之间的关系鲜有研究。本研究旨在探讨中国PD患者中BDNF Val66Met多态性、血浆BDNF水平与焦虑相关特质之间的关系。
这项多中心研究纳入了116例PD患者和99名健康对照者。我们检测了两组中BDNF rs6265（Val66Met）的单核苷酸多态性（SNP）和BDNF血浆水平。此外，对PD患者进行了状态-特质焦虑量表（STAI）、惊恐障碍严重程度量表中文版（PDSS-CV）和汉密尔顿焦虑量表（HAMA-14）评估。对不同基因型受试者的BDNF浓度差异进行定量比较，并分析BDNF Val66Met基因型与特质焦虑之间的关联。
PD患者与健康对照者之间的基因型频率（=0.79）或等位基因频率（=0.88）无显著差异。PD患者的BDNF血浆水平显著低于对照组（=0.003）。PD患者中，Met/Met基因型的BDNF血浆水平显著低于Val/Met基因型（=0.033）。携带Met/Met基因型的PD患者在STAI特质量表上的得分显著高于携带Val/Val基因型（=0.045）和Val/Met基因型（=0.018）的患者。有广场恐怖症的PD患者的STAI特质量表得分显著高于无广场恐怖症的患者（<0.05）。协方差分析显示，因变量STAI特质量表得分受“基因型”因素（Val/Val、Val/Met、Met/Met，=0.029）和协变量“广场恐怖症”（=0.008）的显著影响。在该模型中，BDNF基因型解释了STAI特质量表得分11.5%的变异。对比分析显示，Met/Met受试者的STAI特质量表得分显著高于Val/Met（=0.018）和Val/Val个体（=0.045）。
我们发现焦虑特质与PD患者的BDNF多态性有关。BDNF Met/Met基因型可能会降低PD患者的血浆BDNF水平并增加特质焦虑。
