Yang Yu, Tang Jia, Yang Haiyan, Yang Shuling, Cai Ming, Qi Ao, Lan Xianyong, Huang Bizhi, Su Chao, Chen Hong
Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A & F University, Yangling, China.
Yunnan Academy of Grassland and Animal Science, Kunming, China.
Anim Biotechnol. 2023 Dec;34(7):2141-2149. doi: 10.1080/10495398.2022.2077740. Epub 2022 Jul 10.
Beef production is closely related to the national economy and the attention has been paid to the improvement of beef cattle by molecular markers associated. Copy number variations (CNVs) recently have been gained many researches and recognized as an important source of genetic variation. Extensive studies have indicated that CNVs have effects on a large range of economic traits by a wide range of gene copy number alteration. S100A7 is a member of S100 family which is a famous family of Ca-binding proteins. plays a crucial role in many important phenotypes (progress) including inflammatory diseases, psoriasis, obesity, etc. The aim of our study was to explore the phenotypic effects of CNV located in the gene of bovine chromosome 3. We detected CNV by qPCR in different cattle breeds, including Qinchuan cattle, Yunling cattle, Xianan cattle and a crossbred group Pinan. The copy number was identified as gain, normal and loss type, our results showed that the gain type was the main type in three types of CNV of the whole tested breeds. After CNV detection, association analysis between CNV and growth traits was carried out in four cattle breeds. We found significant effects of the CNV on cattle growth traits with differently preferred CNV types such as gain type with better chest depth ( = 0.043) in QC, loss type with better body length ( = 0.008) and rump width ( = 0.014) in YL, normal with better chest girth ( = 0.001), gain with better waist width ( = 0.001) and rump width ( = 0.044) in PN. These results suggested that the CNV could affect the phenotypic traits and be used as a promising genetic marker for cattle molecular breeding.
牛肉生产与国民经济密切相关，通过相关分子标记改善肉牛的研究已受到关注。拷贝数变异（CNV）近年来得到了许多研究，并被认为是遗传变异的重要来源。大量研究表明，CNV通过广泛的基因拷贝数改变对一系列经济性状产生影响。S100A7是S100家族的成员，S100家族是著名的钙结合蛋白家族。在许多重要表型（进展）中发挥关键作用，包括炎症性疾病、银屑病、肥胖症等。我们研究的目的是探索位于牛3号染色体基因中的CNV的表型效应。我们通过qPCR在不同牛品种中检测CNV，包括秦川牛、云岭牛、西南牛和一个杂交组皮南牛。拷贝数被鉴定为增加型、正常型和缺失型，我们的结果表明，在整个测试品种的三种CNV类型中，增加型是主要类型。在检测到CNV后，对四个牛品种进行了CNV与生长性状的关联分析。我们发现CNV对牛生长性状有显著影响，不同的CNV类型有不同的偏好，如在秦川牛中增加型对胸深更好（P = 0.043），在云岭牛中缺失型对体长更好（P = 0.008）和臀宽更好（P = 0.014），在皮南牛中正常型对胸围更好（P = 0.001），增加型对腰宽更好（P = 0.001）和臀宽更好（P = 0.044）。这些结果表明，该CNV可以影响表型性状，并有望作为牛分子育种的遗传标记。
