College of Animal Science, Xinjiang Agriculture University, Urumqi, Xinjiang 830052, People's Republic of China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
Gene. 2022 Feb 5;810:146060. doi: 10.1016/j.gene.2021.146060. Epub 2021 Nov 2.
Previous, studies have shown that the dynein transporter compound has a role in diseases such as intellectual disability and cerebral malformations. However, the study of CNV in DYNC1I2 gene has not been reported. Q-PCR and data association analysis were used for DYNC1I2 gene copy in this study. In this study, blood samples were collected from five breeds of Chinese cattle (Qingchuan cattle, Xianan cattle, Yunling cattle, Pinan cattle and Guyuan cattle) for DYNC1I2 gene CNV type detection. SPSS 20.0 software and method of ANOVA were used to analyzed the association between types of CNV and growth traits. Results reveal that the distribution of different copy number types in different cattle breeds is different. Association analysis indicate that CNV of DYNC1I2 gene showed a positive effect in cattle growth: in XN cattle, individuals with deletion types showed better performance on height at hip cross (P < 0.05); individuals with duplication types have better performance on body length (P < 0.05) in PN cattle; individuals with deletion types was significantly correlated with chest width and Hucklebone width (P < 0.05) in QC cattle; individuals with duplication types in Yunling cattle were better than the normal types, and there was a significant correlation between copy number variant and chest depth (P < 0.05). The results showed that CNV markers closely related to cattle production traits were detected at DNA level, which could be used as an important candidate molecular marker for marker-assisted selection of growth traits in Chinese cattle, and provided a new research basis for genetics and breeding of Chinese beef cattle.
先前的研究表明，动力蛋白转运复合物在智力障碍和脑畸形等疾病中具有作用。然而，DYNC1I2 基因中的 CNV 研究尚未报道。本研究使用 Q-PCR 和数据关联分析检测了 DYNC1I2 基因的拷贝数。在本研究中，从中国五个牛品种（秦川牛、西安牛、云岭牛、坪山牛和固原牛）采集血液样本，用于检测 DYNC1I2 基因 CNV 类型。使用 SPSS 20.0 软件和方差分析方法分析了 CNV 类型与生长性状之间的关联。结果表明，不同牛品种中不同拷贝数类型的分布不同。关联分析表明，DYNC1I2 基因的 CNV 对牛的生长具有正效应：在 XN 牛中，缺失类型个体在臀高（P<0.05）上表现更好；PN 牛中，重复类型个体在体长（P<0.05）上表现更好；QC 牛中，缺失类型个体与胸宽和髋骨宽显著相关（P<0.05）；云岭牛中，重复类型个体优于正常类型，并且拷贝数变异与胸深之间存在显著相关性（P<0.05）。结果表明，在 DNA 水平上检测到与牛生产性状密切相关的 CNV 标记，可作为中国牛生长性状标记辅助选择的重要候选分子标记，为中国肉牛遗传育种提供了新的研究基础。
