Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, China.
National Chemistry Experimental Teaching Demonstration Center, Jilin University, Jilin, Changchun, China.
Luminescence. 2022 Sep;37(9):1524-1531. doi: 10.1002/bio.4326. Epub 2022 Jul 18.
In this work, a highly efficient electrochemiluminescence (ECL) biosensor was developed based on the nanosponge-hydrogel system for uric acid (UA) detection. First, the nanosponge consisted of polylactic acid glycolic acid (PLGA) nanoparticles immobilized with MoS quantum dots (QDs) and urate oxidase (UAO). The marked loading capability of PLGA nanoparticles enables loading many biomolecules and QDs for the specific recognition of UA. Urate oxidase on the nanosponge can catalyze UA to generate H O in situ, which further triggers the ECL signal for the MoS QDs. Furthermore, the biocompatible acrylamide-based hydrogel not only effectively retained the functionalities of the chimeric nanosponge-hydrogel, but also provided structural integrity and engineering flexibility on the electrode for ECL sensing applications. In addition, there were many ester groups and amide bonds in the nanosponge-hydrogel structure. Therefore, many electron can be excited in the ECL process due to the large number of lone electron pairs on oxygen and nitrogen atoms. This resulted in a seven-fold ECL enhancement of the MoS QDs. Finally, the nanosponge-hydrogel structure-based ECL biosensor was successfully used in real clinical serum assays. This showed a good analytical performance for UA detection (100-500 μmol/L) with a limit of detection of 20 μmol/L.
在这项工作中，基于纳米海绵-水凝胶体系开发了一种用于尿酸（UA）检测的高效电致化学发光（ECL）生物传感器。首先，纳米海绵由聚乳酸-羟基乙酸共聚物（PLGA）纳米粒子固定化的 MoS 量子点（QD）和尿酸氧化酶（UAO）组成。PLGA 纳米粒子的标记负载能力使其能够负载许多生物分子和 QD，用于 UA 的特异性识别。纳米海绵上的尿酸氧化酶可以催化 UA 原位生成 H O，进而触发 MoS QD 的 ECL 信号。此外，基于丙烯酰胺的水凝胶不仅具有良好的生物相容性，而且还为电极上的 ECL 传感应用提供了结构完整性和工程灵活性。此外，纳米海绵-水凝胶结构中存在许多酯基和酰胺键。因此，由于氧和氮原子上大量的孤对电子，在 ECL 过程中可以激发许多电子。这导致 MoS QD 的 ECL 增强了七倍。最后，基于纳米海绵-水凝胶结构的 ECL 生物传感器成功地用于真实的临床血清分析。该传感器对 UA 的检测表现出良好的分析性能（100-500 μmol/L），检测限为 20 μmol/L。
