[血气分析仪测定血红蛋白和血细胞比容的变异性]

[Variability of hemoglobin and hematocrit determined in blood gas equipment].

作者信息

Santos-Martínez Luis Efrén, Cortés-Ruiz Jorge René, Vázquez-Tecpanecatl Olivia, Ordoñez-Reyna Adriana, Quevedo-Paredes Javier

机构信息

Instituto Mexicano del Seguro Social, Centro Médico Nacional Siglo XXI, Hospital de Cardiología, Departamento de Hipertensión Pulmonar y Corazón Derecho. Ciudad de México, México.

Instituto Mexicano del Seguro Social, Centro Médico Nacional "La Raza", Hospital de Especialidades "Antonio Fraga Mouret", Coordinación del Curso Profesional Técnico Universitario en Terapia Respiratoria. Ciudad de México, México.

出版信息

Rev Med Inst Mex Seguro Soc. 2022 Jul 4;60(4):363-370.

PMID:35816622

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10395986/

Abstract

BACKGROUND

Usually hemoglobin and hematocrit are obtained from peripheral venous blood samples processed in equipment for hematic biometry. These parameters can also be determined from arterial samples processed on blood gas equipment. Its variability when using arterial samples and this equipment is unknown, in addition, if the lack of knowledge of this variability affects clinical decisions.

OBJECTIVE

To know the variability of hemoglobin and hematocrit from arterial blood samples processed in blood gas equipment.

MATERIAL AND METHODS

Analytic cross design. Consecutive subjects with stable cardiopulmonary disease were blindly studied. The samples were obtained at the same time for analysis in both equipments. Variability was analyzed with Bland-Altman descriptive statistic and its magnitude with the intraclass correlation coefficient.

RESULTS

Two hundred of blood samples from 50 patients were studied, 31 (62%) women, age 62 ± 14 years old, body mass index 30.11 ± 5.69 Kg/m2. The main comparison was peripheral venous blood sample processed in laboratory equipment (SYSMEX) vs. arterial sample on blood gas equipment (GEM). The mean difference (bias) and intraclass correlation coefficient for hemoglobin were: 0.12 (-1.45, 1.23) and 0.95 (0.91, 0.97); for the hematocrit -4.4 (-0.4, 8.54), and 0.72 (0.51, 0.84).

CONCLUSIONS

The mean difference and bias for hemoglobin was close to 0; the hematocrit was higher. Hemoglobin from arterial samples processed in blood gas equipment can be used to make clinical decisions.

摘要

背景

通常血红蛋白和血细胞比容是通过在血液生物计量设备中处理的外周静脉血样本获得的。这些参数也可以从在血气设备上处理的动脉样本中确定。使用动脉样本和该设备时其变异性尚不清楚，此外，这种变异性的缺乏是否会影响临床决策也不清楚。

目的

了解在血气设备中处理的动脉血样本中血红蛋白和血细胞比容的变异性。

材料与方法

采用分析性交叉设计。对患有稳定心肺疾病的连续受试者进行盲法研究。同时采集样本在两种设备上进行分析。使用布兰德-奥特曼描述性统计分析变异性，并使用组内相关系数分析其大小。

结果

研究了50例患者的200份血样，其中31例（62%）为女性，年龄62±14岁，体重指数30.11±5.69kg/m²。主要比较是在实验室设备（希森美康）中处理的外周静脉血样本与在血气设备（GEM）上处理的动脉样本。血红蛋白的平均差异（偏差）和组内相关系数分别为：0.12（-1.45，1.23）和0.95（0.91，0.97）；血细胞比容为-4.4（-0.4，8.54），以及0.72（0.51，0.84）。