College of Food Science and Engineering, Henan University of Technology, Zhengzhou, 450001, PR China; Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Zhengzhou, Henan, 450001, People's Republic of China.
College of Food Science and Engineering, Henan University of Technology, Zhengzhou, 450001, PR China; Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Zhengzhou, Henan, 450001, People's Republic of China.
Toxicon. 2022 Sep;216:139-147. doi: 10.1016/j.toxicon.2022.06.009. Epub 2022 Jul 8.
The zebrafish model was used to evaluate the antioxidant properties of cyanidin-3-O-glucoside (CG) and its metabolite protocatechuic acid (PCA) against aflatoxin B (AFB)-induced hepatotoxicity and oxidative stress. In this study, zebrafish larvae were cultured for 3 days post fertilization (dpf) and then induced with AFB. After induced 4 h, 8 h, 12 h, and 24 h, 5 μg/mL CG/PCA was added and then co-cultured to 5 dpf, respectively. The experiments showed that CG/PCA suppressed AFB-induced zebrafish liver atrophy and delayed the absorption of the yolk sac. In addition, reactive oxygen species (ROS) and cell death were also significantly decreased by 5 μg/mL CG/PCA (P ˂ 0.05). CG/PCA significantly reduced hepatic biomarkers in the serum contents (P ˂ 0.05). Besides, glutathione (GSH) contents were significantly upregulated, and the activities of superoxide dismutase (SOD) and catalase (CAT) were significantly elevated in zebrafish (P ˂ 0.05). The addition of 5 μg/mL CG/PCA was capable of reducing the apoptotic levels of caspase-9 and caspase-3 after 100 ng/mL AFB intoxication. In conclusion, these results suggested that CG and its metabolite PCA might antagonize the hepatotoxicity of AFB, reduce oxidative damage and inhibit cell death.
斑马鱼模型被用于评估矢车菊素-3-O-葡萄糖苷（CG）及其代谢产物原儿茶酸（PCA）对黄曲霉毒素 B（AFB）诱导的肝毒性和氧化应激的抗氧化特性。在这项研究中，斑马鱼幼虫在受精后 3 天（dpf）进行培养，然后用 AFB 诱导。诱导后 4 h、8 h、12 h 和 24 h 时，分别加入 5 μg/mL CG/PCA 并共同培养至 5 dpf。实验表明，CG/PCA 抑制了 AFB 诱导的斑马鱼肝萎缩，并延缓了卵黄囊的吸收。此外，5 μg/mL CG/PCA 还显著降低了活性氧（ROS）和细胞死亡（P ˂ 0.05）。CG/PCA 还显著降低了血清中肝生物标志物的含量（P ˂ 0.05）。此外，谷胱甘肽（GSH）含量显著上调，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性也显著升高（P ˂ 0.05）。在 100ng/mL AFB 中毒后，添加 5μg/mL CG/PCA 能够降低 caspase-9 和 caspase-3 的凋亡水平。综上所述，这些结果表明 CG 和其代谢产物 PCA 可能拮抗 AFB 的肝毒性，减轻氧化损伤并抑制细胞死亡。
