Sablé-Amplis R, Sicart R, Farré G
Comp Biochem Physiol B. 1987;86(4):725-9. doi: 10.1016/0305-0491(87)90219-7.
The rates of in vitro incorporation of [2-14C]mevalonate and of [2-14C]acetate were determined in the liver and in the ileum obtained from two groups of hamsters. The first (N-H) were conventional hamsters with normal cholesterolemia, the second (FEC-H) were hamsters which develop high levels of cholesterol in plasma and in the liver with age. In FEC-H, the levels of cholesterol reached 130 to 220 mg/100 ml in plasma and 600-2400 mg/100 g fresh tissue in the liver in contrast to about 100 mg and 350 mg respectively in the N-H group. The accumulation of cholesterol in the tissues of FEC-H was found to be associated with a strong decrease in the incorporation rate of the precursors into cholesterol, but above all, the distribution of the radioactivity derived from [2-14C]mevalonate between free cholesterol and esterified cholesterol was markedly different in the two groups of hamsters. In FEC-H, 78% of the radioactivity was found in the esters, as opposed to 10% found in the N-H group. Thus, the development of hypercholesterolemia with age in FEC-H might be related to alterations in the enzyme systems (ACAT, CEH) which modulate the level of cholesterol esters in the liver. No significant difference was observed between the two groups of hamsters either in the concentration of cholesterol or in the rate of cholesterogenesis in the ileum.
测定了两组仓鼠肝脏和回肠中[2-¹⁴C]甲羟戊酸和[2-¹⁴C]乙酸的体外掺入率。第一组（N-H）是胆固醇血症正常的普通仓鼠，第二组（FEC-H）是随着年龄增长血浆和肝脏中胆固醇水平升高的仓鼠。在FEC-H组中，血浆胆固醇水平达到130至220mg/100ml，肝脏中新鲜组织胆固醇水平达到600 - 2400mg/100g，而N-H组分别约为100mg和350mg。发现FEC-H组组织中胆固醇的积累与前体掺入胆固醇的速率大幅降低有关，但最重要的是，两组仓鼠中[2-¹⁴C]甲羟戊酸产生的放射性在游离胆固醇和酯化胆固醇之间的分布明显不同。在FEC-H组中，78%的放射性存在于酯类中，而N-H组为10%。因此，FEC-H组中随着年龄增长出现的高胆固醇血症可能与调节肝脏中胆固醇酯水平的酶系统（ACAT、CEH）改变有关。两组仓鼠在回肠中的胆固醇浓度或胆固醇生成速率方面均未观察到显著差异。
