Environmental and Computational Chemistry Group, School of Pharmaceutical Sciences, Zaragocilla Campus, University of Cartagena, 130014, Cartagena, Colombia.
Center for Chromatography and Mass Spectrometry, CROM-MASS, CIBIMOL-CENIVAM, Industrial University of Santander, Carrera 27, Calle 9, Building 45, 680002, Bucaramanga, Colombia.
BMC Complement Med Ther. 2022 Jul 11;22(1):184. doi: 10.1186/s12906-022-03654-6.
H. brasiletto is used in popular culture due to its therapeutic properties, including antioxidant, anti-inflammatory and antiproliferative properties, although little is known about its role as a protector against metal toxicity. This study aimed to investigate the chemical composition and efficacy of the hydroalcoholic extract from H. brasiletto (HAE-Hbrasiletto) collected in northern Colombia to defend against cadmium (Cd)-induced toxicity.
Phytochemical characterization was performed using HPLC-ESI-QTOF. Caenorhabditis elegans was employed to assess the shielding effect of HAE-Hbrasiletto against Cd toxicity in vivo, and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was utilized to measure radical scavenging activity.
The main secondary metabolites identified by HPLC-ESI-QTOF in the extracts were hematoxylins (brazilein and hematein) and protosappanins (protosappanin A, B and C, 10-O-methylprotosappanin B, and protosappanin A dimethyl acetal). The HAE-Hbrasiletto elicited low lethality in N2 worms and significantly reduced the Cd-induced death of the nematodes. It also improved Cd-induced motility inhibition, as well as body length and reproduction reduction provoked by the heavy metal. The extract displayed a good capacity to halt Cd-induced DAF-16 translocation. As this last process was associated with lethality (r = 0.962, p < 0.01), the antioxidant properties of the extract may contribute to ameliorating tissue damage induced by oxidative stress from Cd exposure.
HAE-Hbrasiletto has remarkable properties to protect against Cd-induced toxicity.
由于具有抗氧化、抗炎和抗增殖等治疗特性，H. brasiletto 在大众文化中被广泛应用，尽管人们对其作为金属毒性保护剂的作用知之甚少。本研究旨在探究产自哥伦比亚北部的 H. brasiletto 水醇提取物（HAE-Hbrasiletto）的化学成分和功效，以研究其抵御镉（Cd）诱导毒性的作用。
采用 HPLC-ESI-QTOF 进行植物化学特征分析。利用秀丽隐杆线虫评估 HAE-Hbrasiletto 对体内 Cd 毒性的屏蔽作用，并采用 2,2-二苯基-1-苦基肼基（DPPH）法测定自由基清除活性。
HPLC-ESI-QTOF 鉴定出提取物中的主要次生代谢物为血根素（巴西苏木素和血根素）和原巴西苏木素（原巴西苏木素 A、B 和 C、10-O-甲基原巴西苏木素 B 和原巴西苏木素 A 二甲缩醛）。HAE-Hbrasiletto 对 N2 线虫的致死率较低，能显著降低 Cd 诱导线虫死亡的作用。它还能改善 Cd 诱导的线虫运动抑制、体长缩短和繁殖减少，这些均由重金属引起。提取物能有效阻止 Cd 诱导的 DAF-16 易位。由于这一最后过程与致死性相关（r = 0.962，p < 0.01），提取物的抗氧化特性可能有助于减轻 Cd 暴露引起的氧化应激导致的组织损伤。
HAE-Hbrasiletto 具有显著的保护作用，可抵御 Cd 诱导的毒性。
