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利用γ-生育酚甲基转移酶和谷氨酸(formimino)转移酶基因进行标记辅助聚合,培育生物强化甜玉米杂交种。

Marker-assisted pyramiding of γ-tocopherol methyltransferase and glutamate formiminotransferase genes for development of biofortified sweet corn hybrids.

机构信息

Institute of Maize and Featured Upland Crops, Zhejiang Academy of Agricultural Sciences, Dongyang, Zhejiang, China.

National Maize Improvement Center of China, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, China.

出版信息

PeerJ. 2022 Jul 6;10:e13629. doi: 10.7717/peerj.13629. eCollection 2022.

DOI:10.7717/peerj.13629
PMID:35818359
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9270877/
Abstract

Micronutrients, including vitamins, minerals, and other bioactive compounds, have tremendous impacts on human health. Much progress has been made in improving the micronutrient content of inbred lines in various crops through biofortified breeding. However, biofortified breeding still falls short for the rapid generation of high-yielding hybrids rich in multiple micronutrients. Here, we bred multi-biofortified sweet corn hybrids efficiently through marker-assisted selection. Screening by molecular markers for vitamin E and folic acid, we obtained 15 inbred lines carrying favorable alleles (six for vitamin E, nine for folic acid, and three for both). Multiple biofortified corn hybrids were developed through crossing and genetic diversity analysis.

摘要

微量营养素,包括维生素、矿物质和其他生物活性化合物,对人类健康有巨大影响。通过生物强化育种,在提高各种作物自交系的微量营养素含量方面已经取得了很大进展。然而,生物强化育种在快速产生富含多种微量营养素的高产品种方面仍然不足。在这里,我们通过标记辅助选择高效地培育了多种生物强化甜玉米杂交种。通过对维生素 E 和叶酸的分子标记进行筛选,我们获得了 15 个携带有利等位基因的自交系(六个用于维生素 E,九个用于叶酸,三个用于两者)。通过杂交和遗传多样性分析,开发了多种生物强化玉米杂交种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/02707697697a/peerj-10-13629-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/39b8203efd92/peerj-10-13629-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/9097e8367a12/peerj-10-13629-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/30bda2410e93/peerj-10-13629-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/02707697697a/peerj-10-13629-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/39b8203efd92/peerj-10-13629-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/9097e8367a12/peerj-10-13629-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/30bda2410e93/peerj-10-13629-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a792/9270877/02707697697a/peerj-10-13629-g004.jpg

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Plant Genome. 2022 Jun;15(2):e20197. doi: 10.1002/tpg2.20197. Epub 2022 Mar 8.
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Natural variation for carotenoids in fresh kernels is controlled by uncommon variants in sweet corn.
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Plant Genome. 2020 Mar;13(1):e20008. doi: 10.1002/tpg2.20008. Epub 2020 Apr 24.
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Molecular Breeding for Nutritionally Enriched Maize: Status and Prospects.营养强化玉米的分子育种:现状与展望
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Genes (Basel). 2019 Dec 28;11(1):37. doi: 10.3390/genes11010037.
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