Liu Yinghui, He Ruyue, Zhu Min, Yu Haijia
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.
Methods Mol Biol. 2022;2473:141-156. doi: 10.1007/978-1-0716-2209-4_11.
The GLUT4 vesicle fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a variety of regulatory proteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion events.
GLUT4囊泡融合由可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNAREs）和多种调节蛋白介导。例如，Synip和Tomosyn对GLUT4 SNARE介导的膜融合起负调节作用。在此，我们描述了体外重组测定法，以确定SNAREs、Synip和Tomosyn的分子机制。这些方法也可扩展到其他类型膜融合事件的研究。
