介导GLUT4囊泡融合的SNARE蛋白及其调节因子的体外重组研究

In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion.

作者信息

Liu Yinghui, He Ruyue, Zhu Min, Yu Haijia

机构信息

Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China.

出版信息

Methods Mol Biol. 2022;2473:141-156. doi: 10.1007/978-1-0716-2209-4_11.

DOI:10.1007/978-1-0716-2209-4_11

PMID:35819764

Abstract

The GLUT4 vesicle fusion is mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and a variety of regulatory proteins. For example, synip and tomosyn negatively regulate GLUT4 SNARE-mediated membrane fusion. Here we describe in vitro reconstituted assays to determine the molecular mechanisms of SNAREs, synip, and tomosyn. These methods can also be extended to the studies of other types of membrane fusion events.

摘要

GLUT4囊泡融合由可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体（SNAREs）和多种调节蛋白介导。例如，Synip和Tomosyn对GLUT4 SNARE介导的膜融合起负调节作用。在此，我们描述了体外重组测定法，以确定SNAREs、Synip和Tomosyn的分子机制。这些方法也可扩展到其他类型膜融合事件的研究。