Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, No. 35 Tsinghua East Road, Beijing 100083, China.
Beijing Key Laboratory of Forestry Food Processing and Safety, Department of Food Science and Engineering, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
J Agric Food Chem. 2022 Jul 27;70(29):9084-9094. doi: 10.1021/acs.jafc.2c00368. Epub 2022 Jul 12.
In this study, four hybrids from the cross between "Italia" and "Tamina" grapes were chosen to investigate their distinct monoterpenoids patterns and candidate genes involved. Monoterpenoid profiles and transcriptome data were generated at four berry developmental stages. Trans-rose oxide, cis-rose oxide, citronellol, neral, nerol, nerol oxide, geraniol, geranial, geranic acid, and cis-isogeraniol were the dominant compounds in R250 hybrid, while linalool, hotrienol, linalool oxide pyranoside, and cis-furan linalool oxide were the main compounds in R77 hybrid. Six TPS-g subfamily genes were found related with the contents of linalool and its related monoterpenoids by weighted gene coexpression network analysis (WGCNA) and phylogenetic analysis. Among them, TPS59 was cloned and functionally verified by transient overexpression in the leaves of . Meanwhile, NAC (), C2C2-GATA (), and bHLH () were selected as candidate transcription factors (TFs) that could regulate the expression of the six TPS-b genes. These data enhanced our understanding on the regulation of monoterpenoid biosynthesis in grapes.
在这项研究中，选择了“Italia”和“Tamina”葡萄杂交的四个杂种，以研究它们独特的单萜化合物模式和涉及的候选基因。在四个浆果发育阶段生成了单萜化合物图谱和转录组数据。反式-玫瑰氧化物、顺式-玫瑰氧化物、香茅醇、橙花醛、橙花醇、橙花醇氧化物、香叶醇、牻牛儿醛、牻牛儿酸和顺式-异牻牛儿醇是 R250 杂种中的主要化合物，而芳樟醇、橙花叔醇、芳樟醇氧化物吡喃糖苷和顺式-呋喃芳樟醇氧化物是 R77 杂种中的主要化合物。通过加权基因共表达网络分析（WGCNA）和系统发育分析，发现了六个与芳樟醇及其相关单萜化合物含量相关的 TPS-g 亚家族基因。其中，TPS59 通过在. 的叶片中瞬时过表达进行了克隆和功能验证。同时，选择 NAC ()、C2C2-GATA () 和 bHLH () 作为候选转录因子（TFs），以调节六个 TPS-b 基因的表达。这些数据增强了我们对葡萄中单萜生物合成调控的理解。
