Zhou Xiaomiao, Liu Songyu, Gao Wengping, Hu Binfang, Zhu Baoqing, Sun Lei
Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China.
Department of Food Science and Engineering, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China.
Plants (Basel). 2022 Aug 18;11(16):2143. doi: 10.3390/plants11162143.
This research investigated the evolution of both monoterpenoids and expression profiles of related biosynthesis genes in the MEP pathway in seven different table grape varieties from veraison to maturity stage in two seasons, and the correlation was further evaluated between monoterpenoid accumulation and expression of these genes studied in these varieties. Results showed that linalool, -furan linalool oxide, geraniol, and -furan linalool oxide were the main compounds in the five Muscat varieties two seasons. 'Zaomeiguixiang' had the highest contents of geraniol and -Citronellol. 'Xiangfei' had the most abundant of linalool and -furan linalool oxide, whereas the neutral varieties of 'Moldova' and 'Christmas Rose' had the least amount. Monoterpenoid volatiles have been grouped in three evolutionary patterns in the berry development of these varieties. 'Zaomeiguixiang' and 'Xiangfei' had distinct different pattern of terpenoids evolution profiles. Pearson's correlation analysis showed that in the MEP pathway, the first biosynthesis gene was significantly correlated to the accumulation of monoterpenoids, and appeared to be an important candidate gene for synthesis of the monoterpenoids.
本研究调查了两个季节中七个不同鲜食葡萄品种从转色期到成熟期单萜类化合物及其在MEP途径中相关生物合成基因表达谱的演变，并进一步评估了这些品种中单萜类化合物积累与所研究基因表达之间的相关性。结果表明，芳樟醇、呋喃型氧化芳樟醇、香叶醇和呋喃型氧化芳樟醇是两个季节中五个玫瑰香品种的主要化合物。‘早美桂香’中香叶醇和香茅醇含量最高。‘香妃’中芳樟醇和呋喃型氧化芳樟醇含量最丰富，而中性品种‘摩尔多瓦’和‘圣诞玫瑰’含量最少。在这些品种的浆果发育过程中，单萜类挥发物已被归为三种进化模式。‘早美桂香’和‘香妃’具有明显不同的萜类化合物进化谱模式。Pearson相关性分析表明，在MEP途径中，第一个生物合成基因与单萜类化合物的积累显著相关，似乎是单萜类化合物合成的一个重要候选基因。
