牛畜牧三个不同研究区域的噬菌体鉴定与特性分析及菌株缺失情况
Identification and Characterization of Phages and Absence of Strains from Three Different Study Areas of Cattle Livestock.
作者信息
Rivera Dácil, Allel Kasim, Salazar Carla, Hamilton-West Christopher, Dueñas Fernando, Hervé Luis Pablo, Moreno-Switt Andrea I
机构信息
Facultad de Ciencias de la Vida, Escuela de Medicina Veterinaria, Universidad Andres Bello, Santiago, Chile.
Facultad de Agronomía y Sistemas Naturales, Facultad de Ciencias Biológicas y Facultad de Medicina, Escuela de Medicina Veterinaria, Pontificia Universidad Católica de Chile, Santiago, Chile.
出版信息
Phage (New Rochelle). 2024 Dec 18;5(4):241-252. doi: 10.1089/phage.2023.0048. eCollection 2024 Dec.
BACKGROUND
represents a significant risk to both public and animal health. Although strains have not been isolated from cattle livestock, phages have been successfully identified.
MATERIALS AND METHODS
Our study was conducted through (i) investigating the presence of strains and phages in three study areas, (ii) phenotypic screening by lytic profile (LP); (iii) selecting nine phages for sequencing; and (iv) genomic comparison to evaluate their relative diversity.
RESULTS
A total of 307 samples were analyzed, resulting in a total of 162 virus-like particles (VLPs) analyzed. The LP was performed to identify strains susceptible to phage infection, including the most frequent serovars: Dublin, Enteritidis, and Javiana. From the VLPs, nine phages were selected for genomic comparison. These phages represent three morphotypes: siphoviruses, myoviruses, and podoviruses, originating from different geographic and productive sites.
CONCLUSIONS
This study enhances the understanding of the presence and diversity of phages in cattle livestock, even in the absence of strains.
背景
对公共卫生和动物健康均构成重大风险。尽管尚未从牛群中分离出[具体菌株名称]菌株，但已成功鉴定出[具体噬菌体名称]噬菌体。
材料与方法
我们的研究通过以下方式进行：（i）调查三个研究区域中[具体菌株名称]菌株和[具体噬菌体名称]噬菌体的存在情况；（ii）通过裂解谱（LP）进行表型筛选；（iii）选择九个噬菌体进行测序；以及（iv）进行基因组比较以评估它们的相对多样性。
结果
共分析了307个样本，总计分析了162个病毒样颗粒（VLP）。通过LP鉴定出易受噬菌体感染的[具体菌株名称]菌株，包括最常见的血清型：都柏林型、肠炎型和贾维亚纳型。从VLP中选择了九个噬菌体进行基因组比较。这些噬菌体代表三种形态类型：长尾噬菌体、肌尾噬菌体和短尾噬菌体，源自不同的地理和生产地点。
结论
本研究增进了对牛群中[具体噬菌体名称]噬菌体的存在情况和多样性的了解，即使在没有[具体菌株名称]菌株的情况下也是如此。
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