Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China.
Anal Chem. 2022 Jul 26;94(29):10524-10530. doi: 10.1021/acs.analchem.2c02004. Epub 2022 Jul 13.
In this work, an intelligent multiregion linear DNA walker (MLDW) with a high walking rate and a high amplification efficiency was explored for ultrasensitive detection of miRNA. Significantly, amounts of functional domain could be concentrated in a long linear DNA obtained by the target miRNA-mediated rolling-circle amplification to simultaneously increase the local concentration and collision probability, resulting in an obviously improved reaction rate. Impressively, the MLDW can accomplish the reaction within 30 min, which is at least 4 times beyond that of traditional single-leg and multiple-leg DNA walkers. As a proof of concept, the high-efficiency MLDW was used to develop an electrochemical biosensing platform for ultrasensitive detection of target miRNA-21 with a low detection limit down to 36 aM. Therefore, the MLDW we designed puts forward an innovative insight to construct a functional DNA nanodevice and promote the investigation of the inherent performance of nucleic acid signal amplification for ultimate application in the detection of biomolecules and clinical disease diagnosis.
在这项工作中，探索了一种具有高行走速率和高扩增效率的智能多区域线性 DNA walker（MLDW），用于超灵敏检测 miRNA。重要的是，通过靶 miRNA 介导的滚环扩增获得的长线性 DNA 中可以集中大量功能域，从而同时增加局部浓度和碰撞概率，从而显著提高反应速率。令人印象深刻的是，MLDW 可以在 30 分钟内完成反应，这至少是传统单腿和多腿 DNA walker 的 4 倍。作为概念验证，高效的 MLDW 被用于开发用于超灵敏检测靶 miRNA-21 的电化学生物传感平台，检测限低至 36 aM。因此，我们设计的 MLDW 为构建功能性 DNA 纳米器件提出了创新性的见解，并促进了对核酸信号扩增固有性能的研究，最终应用于生物分子的检测和临床疾病的诊断。
