Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education Chongqing, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education Chongqing, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.
Biosens Bioelectron. 2025 Jan 1;267:116719. doi: 10.1016/j.bios.2024.116719. Epub 2024 Aug 28.
In this work, an ingenious dual-circle DNA walker (DCDW) with pretty fast walking speed and high amplification efficiency was developed for rapid and ultrasensitive electrochemical detection of microRNA-221 (miRNA-221) related to liver cancer, combined with the toehold-mediated strand-displacement reactions (TSDRs). Impressively, compared with the traditional DNA walker, the DCDW with unique double-stranded interlocked DNA nanostructure not only possesses higher stability, flexibility, and anti-entanglement ability, but also enables more functional domain in a smaller area, thereby enhancing the local concentration, which can greatly improve the working efficiency. As a validation, the electrochemical biosensor realized rapid and ultrasensitive detection of miRNA-221 with a reaction time of 15 min and detection limit down to 1.9 aM, and had been applied in MHCC97L and HeLa cancer cell lysates, thus providing an innovative insight to design intelligent functional interlocked DNA walkers for ultimate application in the construction of biosensing platform and miRNA detection in biological sample.
在这项工作中，开发了一种巧妙的双圆 DNA walker（DCDW），它具有相当快的行走速度和高的扩增效率，可用于快速和超灵敏的电化学检测与肝癌相关的 microRNA-221（miRNA-221），结合了引发链置换反应（TSDRs）。令人印象深刻的是，与传统的 DNA walker 相比，具有独特双链互锁 DNA 纳米结构的 DCDW 不仅具有更高的稳定性、灵活性和抗缠结能力，而且能够在更小的区域内具有更多的功能域，从而提高了局部浓度，这可以大大提高工作效率。作为验证，电化学生物传感器实现了 miRNA-221 的快速和超灵敏检测，反应时间为 15 分钟，检测限低至 1.9 aM，并已应用于 MHCC97L 和 HeLa 癌细胞裂解物，从而为设计智能功能互锁 DNA walker 提供了新的见解，可最终应用于生物传感平台的构建和生物样品中 miRNA 的检测。
