在小鼠L细胞中生产人β-干扰素。
Production of human beta-interferon in mouse L-cells.
作者信息
Schulz R, Krafft H, Piehl G W, Lehmann J
出版信息
Dev Biol Stand. 1987;66:489-93.
PMID:3582770
Abstract
Mouse L-cells, producing glycosilated human beta-interferon constitutively, were cultivated on microcarriers in bioreactors, equipped with a bubble-free aeration system up to 22 1 scale. Suitable aeration membrane surfaces and medium exchange rates permitted cultivation up to 8 X 10(6) cells/ml with a specific beta-interferon productivity of 2 X 10(4) U/10(6) cells/day. A correlation was found between the number of cell layers on the microcarriers and the specific human beta-interferon productivity.
摘要
持续产生糖基化人β-干扰素的小鼠L细胞在生物反应器中的微载体上培养，该生物反应器配备了高达22升规模的无泡曝气系统。合适的曝气膜表面和培养基更换率允许培养至8×10⁶个细胞/毫升，β-干扰素的比生产率为2×10⁴单位/10⁶个细胞/天。发现微载体上的细胞层数与特定的人β-干扰素生产率之间存在相关性。
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