在小鼠L细胞中生产人β-干扰素。
Production of human interferon-beta in mouse L-cells.
作者信息
Delzer J, Hauser H, Lehmann J
出版信息
Dev Biol Stand. 1985;60:413-9.
PMID:3899798
Abstract
A mouse fibroblast cell-constructed by genetic engineering--expresses glycosylated human interferon-beta constitutively. The cells were cultivated on microcarriers in special bioreactors. Under these conditions they produce a maximum yield of 6 000 units interferon-beta/ml/day at cell densities of about 2,2 X 10(6) cells/ml. The production stage of interferon-beta is longer than one month.
摘要
一种通过基因工程构建的小鼠成纤维细胞持续表达糖基化的人β干扰素。这些细胞在特殊生物反应器中的微载体上培养。在这些条件下，当细胞密度约为2.2×10⁶个细胞/毫升时，它们每天每毫升可产生最高产量达6000单位的β干扰素。β干扰素的生产阶段超过一个月。
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