Jungbauer A, Wenisch E, Steindl F, Himmler G, Reiter S, Rüker F, Wagner K, Katinger H
Dev Biol Stand. 1987;66:87-90.
Fetal Calf Serum (FCS) was extensively extracted by cross flow diafiltration (Pellicon, Millipore) and sterilized using the basal growth medium (DMEM) for the extraction. Ultrafiltration membranes of 10(5) and 3 X 10(5) Dalton cut off were used respectively. The diafiltrates were used for hybridoma cultivation and the results of growth and mAb-production were compared with standard medium (DMEM + 5% FCS). Slightly reduced mAb-titers were achieved. These were, however, compensated by decreased concentration of contaminating protein and higher specific mAb/protein ratio as examined by SDS-PAGE and enzyme linked immuno electro transfer blot (EITB).
胎牛血清（FCS）通过错流渗滤（Pellicon，密理博公司）进行广泛提取，并使用基础生长培养基（DMEM）进行提取过程中的灭菌处理。分别使用截留分子量为10(5)和3×10(5)道尔顿的超滤膜。渗滤液用于杂交瘤培养，并将生长和单克隆抗体产生的结果与标准培养基（DMEM + 5% FCS）进行比较。单克隆抗体滴度略有降低。然而，通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和酶联免疫电转印（EITB）检测发现，污染蛋白浓度降低以及单克隆抗体/蛋白比例提高，弥补了滴度的下降。
