National Key Laboratory of Crop Genetic Improvement, Ministry of Agriculture Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.
Planta. 2022 Jul 13;256(2):39. doi: 10.1007/s00425-022-03956-6.
This study suggests that stomatal and leaf structures are highly correlated, and mesophyll cell size is an important anatomical trait determining the coordination between stomatal size and mesophyll porosity. A comprehensive study of the correlations between the structural traits and on their relationships with gas exchange parameters may provide some useful information into leaf development and improvement in efficiencies of photosynthetic CO fixation and transpirational water loss. In the present study, nine plant materials from eight crop species were pot grown in a growth chamber. Leaf structural traits, gas exchange, and leaf nitrogen content were measured. We found that stomatal size, mesophyll cell size (MCS), and mesophyll porosity were positively correlated and that the surface areas of mesophyll cells and chloroplasts facing intercellular air spaces were positively correlated with both stomatal density and stomatal area per leaf area (SA). These results suggested that the developments of stomata and mesophyll cells are highly correlated among different crop species. Additionally, MCS was positively correlated with leaf thickness and negatively correlated with leaf density and leaf mass per area, which indicated that MCS might play an important role in leaf structural investments and physiological functions among species. In summary, this study illustrates the correlations between stomatal and mesophyll structures, and it highlights the importance of considering the covariations among leaf traits with the intent of improving photosynthesis and iWUE.
本研究表明，气孔和叶片结构高度相关，且叶肉细胞大小是决定气孔大小与叶肉间隙协调性的重要解剖学特征。对结构特征之间的相关性及其与气体交换参数的关系进行综合研究，可能为叶片发育以及提高光合作用 CO2 固定和蒸腾失水效率提供一些有用信息。本研究中，我们将来自 8 个作物物种的 9 种植物材料在生长室内盆栽种植，测量了叶片结构特征、气体交换和叶片氮含量。我们发现，气孔大小、叶肉细胞大小（MCS）和叶肉间隙率呈正相关，且叶肉细胞和叶绿体的比表面积与气孔密度和比叶面积的气孔面积（SA）呈正相关。这些结果表明，不同作物物种的气孔和叶肉细胞发育高度相关。此外，MCS 与叶片厚度呈正相关，与叶片密度和比叶面积质量呈负相关，这表明 MCS 可能在物种间的叶片结构投资和生理功能中发挥重要作用。总之，本研究说明了气孔和叶肉结构之间的相关性，并强调了在提高光合作用和 iWUE 时考虑叶片特征之间的协变的重要性。
