Aquaculture Biotechnology Research Group, Department of Environmental Science, School of Science, Auckland University of Technology, Private Bag 92006, Auckland, 1142, New Zealand.
NTT Hi-Tech Institute, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam.
Metabolomics. 2022 Jul 13;18(7):52. doi: 10.1007/s11306-022-01907-6.
The Chatham Islands has some of the most prized black-footed abalone (Haliotis iris) beds in New Zealand. This well-managed fishery includes restrictions on catch and size limits, selective fishing methods, and shellfish management. However, recent declines in biomass and growth parameters have prompted omics research to characterise the biological responses of abalone, potentially contributing towards animal management strategies.
The aim of this study was to characterise the metabolite profiles of slow and fast growing, juvenile and adult abalone, relating to metabolites supporting energy metabolism.
A gas chromatography-mass spectrometry metabolite profiling, applying methyl chloroformate alkylation, was performed on juvenile and adult abalone samples collected from Point Durham and Wharekauri sites, Chatham Islands, New Zealand.
The results obtained from haemolymph and muscle samples indicated that abalone from the fast-growing area, Wharekauri, fuelled metabolic functions via carbohydrate sources, providing energy for fatty acid and amino acid synthesis. Conversely, higher amino acid levels were largely utilised to promote growth in this population. The metabolism of juvenile abalone favoured anabolism, where metabolites were diverted from glycolysis and the tricarboxylic acid cycle, and used for the production of nucleotides, amino acids and fatty acids.
This research provides unique physiological insights towards abalone populations supporting the use of metabolomics as a tool to investigate metabolic processes related to growth. This work sets the stage for future work aimed at developing biomarkers for growth and health monitoring to support a growing and more sustainably abalone fishery.
查塔姆群岛拥有新西兰最珍贵的黑足鲍（Haliotis iris）床之一。这个管理良好的渔业包括对捕捞量和尺寸限制、选择性捕捞方法和贝类管理的限制。然而，最近生物量和生长参数的下降促使组学研究对鲍鱼的生物反应进行特征描述，这可能有助于制定动物管理策略。
本研究的目的是描述生长缓慢和快速的幼年和成年鲍鱼的代谢物谱，与支持能量代谢的代谢物有关。
对来自查塔姆群岛达勒姆角和惠雷卡乌里的幼鲍和成年鲍样本进行气相色谱-质谱代谢物图谱分析，采用氯甲酸甲酯烷基化法。
来自血液和肌肉样本的结果表明，来自生长较快的惠雷卡乌里地区的鲍鱼通过碳水化合物来源为代谢功能提供燃料，为脂肪酸和氨基酸合成提供能量。相比之下，这个种群中较高的氨基酸水平主要用于促进生长。幼鲍的新陈代谢有利于合成代谢，代谢物从糖酵解和三羧酸循环中转移出来，用于核苷酸、氨基酸和脂肪酸的生成。
这项研究为支持将代谢组学作为研究与生长相关代谢过程的工具的鲍鱼种群提供了独特的生理见解。这项工作为未来旨在开发生长和健康监测生物标志物以支持不断增长和更可持续的鲍鱼渔业的工作奠定了基础。
