Key Laboratory of Bioengineering Science and Technology, Chongqing University, Ministry of Education, Chongqing, China.
Bioengineering College, Campus B, Chongqing University, Chongqing, People's Republic of China.
PLoS Genet. 2022 Jul 13;18(7):e1010285. doi: 10.1371/journal.pgen.1010285. eCollection 2022 Jul.
During evolutionary adaptation, the mechanisms for self-regulation are established between the normal growth and development of plants and environmental stress. The phytohormone jasmonate (JA) is a key tie of plant defence and development, and JASMONATE-ZIM DOMAIN (JAZ) repressor proteins are key components in JA signalling pathways. Here, we show that JAZ expression was affected by leaf senescence from the transcriptomic data. Further investigation revealed that SlJAZ10 and SlJAZ11 positively regulate leaf senescence and that SlJAZ11 can also promote plant regeneration. Moreover, we reveal that the SlJAV1-SlWRKY51 (JW) complex could suppress JA biosynthesis under normal growth conditions. Immediately after injury, SlJAZ10 and SlJAZ11 can regulate the activity of the JW complex through the effects of electrical signals and Ca2+ waves, which in turn affect JA biosynthesis, causing a difference in the regeneration phenotype between SlJAZ10-OE and SlJAZ11-OE transgenic plants. In addition, SlRbcs-3B could maintain the protein stability of SlJAZ11 to protect it from degradation. Together, SlJAZ10 and SlJAZ11 not only act as repressors of JA signalling to leaf senescence, but also regulate plant regeneration through coordinated electrical signals, Ca2+ waves, hormones and transcriptional regulation. Our study provides critical insights into the mechanisms by which SlJAZ11 can induce regeneration.
在进化适应过程中，植物的正常生长发育与环境胁迫之间建立了自我调节机制。茉莉酸（JA）是植物防御和发育的关键纽带，茉莉酸 ZIM 结构域（JAZ）抑制蛋白是 JA 信号通路的关键组成部分。在这里，我们从转录组数据中表明 JAZ 的表达受叶片衰老的影响。进一步的研究表明，SlJAZ10 和 SlJAZ11 正向调控叶片衰老，SlJAZ11 还可以促进植物再生。此外，我们揭示了 SlJAV1-SlWRKY51（JW）复合物可以在正常生长条件下抑制 JA 的生物合成。受伤后，SlJAZ10 和 SlJAZ11 可以通过电信号和 Ca2+波的作用调节 JW 复合物的活性，进而影响 JA 的生物合成，导致 SlJAZ10-OE 和 SlJAZ11-OE 转基因植物再生表型的差异。此外，SlRbcs-3B 可以维持 SlJAZ11 的蛋白质稳定性，防止其降解。总之，SlJAZ10 和 SlJAZ11 不仅作为 JA 信号通路到叶片衰老的抑制剂，而且通过协调的电信号、Ca2+波、激素和转录调控来调节植物再生。我们的研究为 SlJAZ11 诱导再生的机制提供了重要的见解。
